Evaluation of Platelet Rich Plasma in Skin Graft Take for Patients With Post Burn Raw Areas

Not Applicable

Not yet recruiting

• Conditions: Skin Burn
• Interventions: Procedure: PRP in skin grafts; Procedure: conventional methods in skin grafts

• Registration Number: NCT05996614
• Lead Sponsor: Assiut University

Brief Summary

1. To evaluate the effect of the platelet rich plasma on the take of split thickness skin graft and its early and late results in resurfacing of post burn raw areas.

2. To evaluate the effect of PRP on wound healing on skin graft donor site.

Detailed Description

Burn injuries involve tissue destruction due to energy transfer,The burn injuries can be caused by heat from hot liquids, solids or fire, an less common causes are friction, heat, chemical or electric sources The treatment of burns is complex due to the combination of hypermetabolic physiological response and the need for good functional and cosmetic recovery. Split thickness skin grafts (STSGs) are commonly used to resurface large areas of Post burn raw areas.

The time to complete wound healing ranged from 2 to 75 days. Factors that significantly affected time to complete wound healing were graft loss by seroma, presence of hypergranulation tissue, premorbid conditions, and total body surface area burned.

Generally, skin graft undertakes three steps for healing: anchorage, inosculation, and maturation. Haematoma, shearing, and infection on recipient sites of skin graft can reduce skin graft take, and even lead to skin graft loss.

Platelet-rich plasma (PRP) is an autologous blood-derived biomaterial that is enriched with a 2- to 6-fold concentration of platelets in comparison to normal human platelet concentration. Multiple growth factors and bioactive proteins are stored in α-granules of platelets. These growth factors can promote proliferation of endothelial cells, epithelial cells as well as fibroblasts, and increase angiogenesis. The potential clinical application of PRP in skin graft might rely on its haemostasis, adhesive, antibacterial and healing properties.

Study Timeline

• Study First Submitted: 2022-10-07
• Status Verified: 2023-08
• Study First Submitted QC: 2023-08-10
• Last Update Submitted: 2023-08-10
• Study First Posted: 2023-08-18
• Last Update Posted: 2023-08-18
• Study Start: 2023-10-01
• Primary Completion: 2024-12
• Study Completion: 2025-02

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 40

Inclusion Criteria

1. Age (15- 60 years).
2. Post burn Raw area less than 10 % of total body surface area .
3. Selected donor sites.

Exclusion Criteria

1. Raw area due to other causes ( trauma, ischemia,..etc)
2. Patients with bleeding tendency.
3. Patients with comorbidities(diabetes, malignancy,..etc)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
group with PRP	PRP in skin grafts	Group of patients with post burn raw areas treated with application of platelet rich plasma
group without PRP	conventional methods in skin grafts	Group of patients with post burn raw areas treated with conventional methods

Primary Outcome Measures

Name	Time	Method
histopathological biopsy from both groups by punch Biopsy (vancouver scale )	3 months	pigmentation (0-2) ,normal 0, Hypopigmentation 1 ,Hyperpigmentation 2 Vascularity (0-3) ,Normal 0, Pink 1, Red 2, Purple 3 Pliability (0-5) Normal 0 ,Supple 1 ,Yielding 2,Firm 3, Banding 4, Contracture 5 Height (0-3) Normal(flat)0 ,(0-2mm)1,(2-5mm)2,(\>5mm)3