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Validation of Progranulin as a Biomarker for Sepsis

Completed
Conditions
Sepsis
Registration Number
NCT03280576
Lead Sponsor
Ludwig-Maximilians - University of Munich
Brief Summary

Progranulin blood concentrations in patients with sepsis will be analysed in relation to disease status in order to validate progranulin as a biomarker for sepsis. Patients undergoing cardiac surgery will serve as controls.

Detailed Description

Single nucleotide polymorphisms with known effects on Progranulin plasma concentrations will be assessed in a separate analysis. Expression levels of microRNAs isolated from plasma, circulating exosomes and blood cells will be determined by next-generation sequencing to characterize epigenetic influences on progranulin plasma levels.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
556
Inclusion Criteria
  • Sepsis or Septic Shock defined according to SEPSIS-3 criteria - Age > 18 years
Exclusion Criteria
  • No informed consent
  • Age < 18 years
  • Pregnancy
  • Immunosuppression (including transplantation)
  • Charlson Comorbidity Index > 0 (healthy volunteers)

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Change in progranulin plasma concentrations during ICU - therapyICU admission, day 1, day 4 of ICU therapy

Blood samples will be taken at the abovementioned time points

Secondary Outcome Measures
NameTimeMethod
Change in miRNA expression levels in exosomes, serum and blood cells during ICU - therapyICU admission, day 1, day 4 of ICU therapy

Blood samples will be taken at the abovementioned time points

Trial Locations

Locations (2)

Department of Anesthesiology, Klinikum Neuperlach

🇩🇪

Munich, Bavaria, Germany

Department of Anesthesiology, LMU Munich

🇩🇪

Munich, Bavaria, Germany

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