Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage

Not Applicable

• Conditions: Sleep; Sleep Deprivation
• Interventions: Other: Sleep deprivation; Other: Normal sleep

• Registration Number: NCT02082600
• Lead Sponsor: Federal University of São Paulo

Brief Summary

The aims of this study are examine the effects of sleep deprivation in muscle recovery after a maximum eccentric resistance exercise session performed on an isokinetic dynamometer (24 series of 10 repetitions). The sample will consist of 10 men, sedentary, clinically healthy, aged between 20 and 31 years old. Two experimental groups will be developed: EXE-SLEEP, in which subjects will perform the exercise protocol (\~18:00-19:00) and will be subject to normal period of sleep for 3 nights; EXE-TOTAL, in which subjects will perform the same exercise protocol and will be sleep deprived for 60 hours, followed by one night of sleep rebound.

Detailed Description

The blood parameters analyzed will be:

* Creatine kinase and myoglobin: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol.

* Total and free testosterone, IGF-1, and cortisol: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. Moreover, from 19:00 of the second day to 19:00 of the third day it will be collected blood samples every 2 hours.

* GH: during the second night, GH will be evaluated every 1 hour, from 23:00 to 07:00.

* TNF-alfa, IL-6, IL-1 beta, IL-10, and IL-1ra: before, immediately after, and 2 hour after exercise, as well every 4 hours from 19:00 of the second day to 19:00 of the third day.

It will be evaluated isometric strength every 12 hours, as well visual analog scale of pain.

Study Timeline

• Study Start: 2013-07
• Primary Completion: 2013-12
• Status Verified: 2014-03
• Study First Submitted: 2014-03-06
• Study First Submitted QC: 2014-03-06
• Study First Posted: 2014-03-10
• Last Update Submitted: 2014-03-10
• Last Update Posted: 2014-03-11

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Male
• Target Recruitment: 10

Inclusion Criteria

• healthy subjects
• sedentary
• sleep duration between 6-8 hours peer night

Exclusion Criteria

• smokers
• use vitamin supplements, like C and E
• drink two doses of alcohol/day or > 4 doses in one occasion
• use of anti-inflammatory drugs
• antilipidemic drugs
• anemia
• muscle or joint diseases
• cardiovascular diseases
• diabetes
• renal diseases
• sleep disturbances
• breathing disorders

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Sleep deprivation	Sleep deprivation	Exercise induced-muscle damage protocol followed by 60h of sleep deprivation (two nights) and one night of normal sleep (rebound).
Normal sleep	Normal sleep	Exercise induced-muscle damage protocol followed by 3 nights of normal sleep

Primary Outcome Measures

Name	Time	Method
Change from baseline in isometric strength	Before exercise, immediatly after, 12, 24, 36, 48, and 60 hours after exercise induced-muscle damage

Secondary Outcome Measures

Name	Time	Method
Area Under Curve for serum total and free testosterone, IGF-1, and cortisol	Blood samples will be collected every 2 hours, for 24 hours.

Trial Locations

Locations (1)

Associação Fundo de Incentivo à Pesquisa; 🇧🇷 Sao Paulo, Brazil