Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Change from baseline in isometric strength
研究概览
简要总结
The aims of this study are examine the effects of sleep deprivation in muscle recovery after a maximum eccentric resistance exercise session performed on an isokinetic dynamometer (24 series of 10 repetitions). The sample will consist of 10 men, sedentary, clinically healthy, aged between 20 and 31 years old. Two experimental groups will be developed: EXE-SLEEP, in which subjects will perform the exercise protocol (~18:00-19:00) and will be subject to normal period of sleep for 3 nights; EXE-TOTAL, in which subjects will perform the same exercise protocol and will be sleep deprived for 60 hours, followed by one night of sleep rebound.
详细描述
The blood parameters analyzed will be:
- Creatine kinase and myoglobin: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol.
- Total and free testosterone, IGF-1, and cortisol: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. Moreover, from 19:00 of the second day to 19:00 of the third day it will be collected blood samples every 2 hours.
- GH: during the second night, GH will be evaluated every 1 hour, from 23:00 to 07:00.
- TNF-alfa, IL-6, IL-1 beta, IL-10, and IL-1ra: before, immediately after, and 2 hour after exercise, as well every 4 hours from 19:00 of the second day to 19:00 of the third day.
It will be evaluated isometric strength every 12 hours, as well visual analog scale of pain.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 31 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •healthy subjects
- •sedentary
- •sleep duration between 6-8 hours peer night
排除标准
- •use vitamin supplements, like C and E
- •drink two doses of alcohol/day or > 4 doses in one occasion
- •use of anti-inflammatory drugs
- •antilipidemic drugs
- •muscle or joint diseases
- •cardiovascular diseases
- •renal diseases
- •sleep disturbances
- •breathing disorders
结局指标
主要结局
Change from baseline in isometric strength
时间窗: Before exercise, immediatly after, 12, 24, 36, 48, and 60 hours after exercise induced-muscle damage
次要结局
- Area Under Curve for serum total and free testosterone, IGF-1, and cortisol(Blood samples will be collected every 2 hours, for 24 hours.)
研究者
Hanna Karen Moreira Antunes
PhD
Federal University of São Paulo