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Post Transplant Cyclophosphamide (PTCY) as Sole Graft Versus Host Disease (GVHD) Prophylaxis for Matched Allotransplant: CYRIC

Phase 2
Terminated
Conditions
Graft Versus Host Disease
Interventions
Radiation: Full body irradiation
Other: stem cell transplantation
Other: nuclear cells
Registration Number
NCT03263767
Lead Sponsor
Nantes University Hospital
Brief Summary

Acute or chronic graft versus host disease is still the major complication of stem cells transplantation regarding morbidity and mortality.

Recently, high dose cyclophosphamide utilization early after post-transplantation (day+ 3 and +4) not only for patients with HLA- haploidentical donor but also for patients with Human Leukocyte Antigen (HLA)-compatible donor, showed a great control of graft versus host disease after transplantation, allowing to consider stopping immunosuppressive treatment after the transplantation (Neoral=cyclosporine, cell-cept=mycophenolate mofetil). Indeed, this step has already been completed in myeloablative transplantation in adult patients.

This approach could enable to avoid in the end several complications related to long term immunosuppressive drugs administration, while promoting quicker immunity recovery.

Detailed Description

The BALTIMORE conditioning regiment will be used in this study with peripheral stem cell transplantation and fludarabine will be replaced by clofarabine for myeloid diseases (Acute Myeloide Leukemia, Myelodysplasia , myelofibrosis, Chronic Myeoloid Leukemia..) because of better antitumoral activity in this setting.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
47
Inclusion Criteria
  • adults ≤ 70 years old
  • indication to stem cells transplantation with reduced-intensity conditioning regimen
  • with a HLA-compatible familial 10/10 or non-familial donor
  • Written signed informed consent form
  • woman with childbearing potential under efficient control birth method during the trial and up to 12 months after cyclophosphamide stop
  • men under efficient control birth method during the trial and up to 6 months after cyclophosphamide stop
  • Negative serology to B and C hepatitis and to HIV
  • Affiliated to social security
Exclusion Criteria
    • Eligible to myeloablative contioning regimen
  • Other progressive malignancy disease or history of prior other malignancy in the last two years, with the exception of: curatively treated basal cell carcinoma or carcinoma in situ of the cervix

  • Progressive mental illness disease

  • Pregnant or Breastfeeding woman

  • woman with childbearing potential without any efficient control birth

  • Serious concomitant infection and not controlled

  • Contra-indications to allogenic transplantation, especially:

    • Cardiac: left ventricular ejection fraction <45% assessed by transthoracic echography or isotopic method (isotopic gamma-angiography)
    • Respiratory: DLCO limiting fludarabine and busulfan use (DLCO< 40% of theorical value)
    • Renal: creatinine clearance < 60ml/min (MDRD method)
    • Hepatic: transaminases >5 Uper Per Normal (UPN) or bilirubin> 2 UPN
  • Contra-indications to cyclophosphamide:

    • Urinary tract infections
    • Acute urothelial toxicity due to cytotoxic chemotherapy or to radiotherapy
    • Obstruction of urines flow
    • Pre-existing hemorrhagic cystitis
    • Yellow fever vaccination
  • Cardiac condition preventing high dose cyclophosphamide utilization :

    • New York Heart Association (NYHA) functional class II, III or IV
    • Rhythmic, valvular or ischemic cardiomyopathy
  • Minor

  • Patient under guardianship or curatorship

  • Patient under judicial protection

  • Known or suspected hypersensitivity to cyclophosphamide

  • Known or suspected hypersensitivity to rabbit proteins

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
LYMPHOID HEMOPATHY without ATGFludarabinepatients with lymphoid hemopathy
LYMPHOID HEMOPATHY without ATGFull body irradiationpatients with lymphoid hemopathy
LYMPHOID HEMOPATHY without ATGstem cell transplantationpatients with lymphoid hemopathy
LYMPHOID HEMOPATHY without ATGnuclear cellspatients with lymphoid hemopathy
MYELOID HEMOPATHY without ATGClofarabinepatients with myeloid hemopathy
MYELOID HEMOPATHY without ATGFull body irradiationpatients with myeloid hemopathy
MYELOID HEMOPATHY without ATGCyclophosphamidepatients with myeloid hemopathy
MYELOID HEMOPATHY without ATGstem cell transplantationpatients with myeloid hemopathy
MYELOID HEMOPATHY without ATGnuclear cellspatients with myeloid hemopathy
LYMPHOID HEMOPATHY witH ATGFull body irradiationpatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
LYMPHOID HEMOPATHY witH ATGstem cell transplantationpatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
LYMPHOID HEMOPATHY witH ATGnuclear cellspatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
MYELOID HEMOPATHY with ATGFull body irradiationpatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
MYELOID HEMOPATHY with ATGstem cell transplantationpatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
MYELOID HEMOPATHY with ATGnuclear cellspatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
LYMPHOID HEMOPATHY witH ATGThymoglobulin Injectable Productpatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
MYELOID HEMOPATHY with ATGThymoglobulin Injectable Productpatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
LYMPHOID HEMOPATHY without ATGCyclophosphamidepatients with lymphoid hemopathy
LYMPHOID HEMOPATHY witH ATGFludarabinepatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
LYMPHOID HEMOPATHY witH ATGCyclophosphamidepatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
MYELOID HEMOPATHY with ATGClofarabinepatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
MYELOID HEMOPATHY with ATGCyclophosphamidepatients inclued after 14 dec 2020, received a conditionnement regimen with ATG on Day -2 to reduce GVHD GRADE 1-2 incidence
Primary Outcome Measures
NameTimeMethod
Incidence of grade 3 and 4 acute GVHD cortico-resistant100 days after transplantation

acute GVHD will be evaluated from International Mount Sinai criteria

Secondary Outcome Measures
NameTimeMethod
graft and relapse free survivalone year

time between Day O and relapse

Infections frequencyone year

time of occurring and frequency of viral (CytoMegalo Virus, Epstein Barr virus , BKV, adenovirus), bacterial, parasite and yeast infections, evaluated by Polymerase Chain Reaction (PCR), blood and urines cultures, biopsy if applicable

Engraftmentone year

chimerism

disease free survival (DFS)one year, the last follow-up visit

blood and bone marrow analysis

Overall survival (OS)one year, the last follow-up visit

clinical follow-up

Chimerism1, 2, 3, 6 and 12 months after transplantation

proportion of full and mixed donor chimerism

Identification of ghost factors associated with GVHDone year

Statistical Models to Identify Subjects with Ghost Prognosis Factors Nguyen JM. 2015

non relapse mortality (NRM)last follow-up visit

number of death unrelated to relapse or disease progression

Immune reconstitution3, 6 and 12 months after transplantation

lymphocytes, monocytes, T4, T8, Natural Killer (NK), B cells rates

Adverse events of grade 3 and 4 after transplantationone year

time of occurring and frequency of grade 3 and grade 4 adverse events (CTCAE criteria)

chronic GVHDone year

chronic GVHD will be assessed with NCI criteria for evaluation of chronic GVHD

compare OS between patients with ATG and patients without ATGone year, last follow-up visit

OS

compare grade 2-4 and 3-4 acute GVHD between patients with ATG and patients without ATGone year

acute GVHD will be evaluated from International Mount Sinai criteria

compare chronic GVHD between patients with ATG and patients without ATGone year

chronic GVHD will be assessed with NCI criteria for evaluation of chronic GVHD

compare DFS between patients with ATG and patients without ATGone year, last follow-up visit

DFS

compare Relapse between patients with ATG and patients without ATGone year, last follow-up visit

Relapse

compare NRM between patients with ATG and patients without ATGone year, last follow-up visit

NRM

compare Infections frequency between patients with ATG and patients without ATGone year

time of occurring and frequency of viral (CytoMegalo Virus, Epstein Barr virus , BKV, adenovirus), bacterial, parasite and yeast infections, evaluated by Polymerase Chain Reaction (PCR), blood and urines cultures, biopsy if applicable

Trial Locations

Locations (1)

Nantes Uh

🇫🇷

Nantes, France

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