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Human Papilloma Virus Vaccine Immunogenicity and Safety Trial in Young Adult Women With GSK Bio's Novel HPV Vaccine.

Phase 2
Completed
Conditions
Infections, Papillomavirus
Interventions
Biological: HPV-16/18/31/45 L1 AS04 Formulation 3
Biological: HPV-16/18/31/45 L1 AS04 Formulation 4
Biological: HPV-16/18/31/45 L1 AS04 Formulation 6
Biological: HPV 16/18 L1 AS04
Biological: HPV-16/18/31/45 L1 AS04 Formulation 1
Biological: HPV-16/18/31/45 L1 AS04 Formulation 2
Biological: HPV-16/18/31/45 L1 AS04 Formulation 5
Registration Number
NCT00231413
Lead Sponsor
GlaxoSmithKline
Brief Summary

Human Papilloma viruses (HPV) are viruses that cause infections of the skin and genitals in men and women. Several types of HPV infection are transmitted by sexual activity and, in women, can infect the cervix (part of the uterus or womb). This infection, if it persists, can lead over a long period of time to cancer of the cervix in women. In collaboration with MedImmune Inc., GlaxoSmithKline Biologicals has developed a HPV vaccine against the oncogenic types HPV-16 and HPV-18 formulated with the adjuvant AS04. GSK Biologicals is also evaluating novel HPV vaccine formulations.This study will evaluate the immunogenicity and safety of a novel GSK Biologicals HPV vaccine in women 18-25 years of age at study start. Approximately 376 study subjects will receive the novel HPV vaccine or the control vaccine administered intramuscularly according to a 0-1-6 month schedule.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
383
Inclusion Criteria
  • A woman between, and including, 18 and 25 years of age at the time of the first vaccination
  • Written informed consent from the subject prior to enrolment
  • Subject must be free of obvious health problems
  • Subject must be of non-childbearing potential and have had no more than 6 lifetime sexual partners
Exclusion Criteria
  • Pregnant or breastfeeding
  • A woman planning to become pregnant or planning to discontinue contraceptive precautions during approximately the first nine months of the study (Month 0-8)
  • Known acute or chronic, clinically significant pulmonary, cardiovascular, neurologic, hepatic or renal functional abnormality
  • History of chronic condition(s) requiring treatment such as cancer, chronic hepatitis or kidney disease(s), diabetes, or autoimmune disease
  • Previous vaccination against human papillomavirus (HPV)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
HPV-TETRA C GroupHPV-16/18/31/45 L1 AS04 Formulation 3Female subjects who received 3 doses of the HPV-16/18/31/45 L1/AS04 vaccine, formulation 3 intramuscularly, into the deltoid of the non-dominant arm according to a 3-dose 0, 1, 6-month vaccination schedule.
HPV-TETRA D GroupHPV-16/18/31/45 L1 AS04 Formulation 4Female subjects who received 3 doses of the HPV-16/18/31/45 L1/AS04 vaccine, formulation 4 intramuscularly, into the deltoid of the non-dominant arm according to a 3-dose 0, 1, 6-month vaccination schedule.
HPV-TETRA F GroupHPV-16/18/31/45 L1 AS04 Formulation 6Female subjects who received 3 doses of the HPV-16/18/31/45 L1/AS04 vaccine, formulation 6 intramuscularly, into the deltoid of the non-dominant arm according to a 3-dose 0, 1, 6-month vaccination schedule.
HPV-16/18 GroupHPV 16/18 L1 AS04Female subjects who received 3 doses of the HPV-16/18 L1 AS04 vaccine intramuscularly, into the deltoid of the non-dominant arm according to a 3-dose 0, 1, 6-month vaccination schedule.
HPV-TETRA A GroupHPV-16/18/31/45 L1 AS04 Formulation 1Female subjects who received 3 doses of the HPV-16/18/31/45 L1/AS04 vaccine, formulation 1 intramuscularly, into the deltoid of the non-dominant arm according to a 3-dose 0, 1, 6-month vaccination schedule.
HPV-TETRA B GroupHPV-16/18/31/45 L1 AS04 Formulation 2Female subjects who received 3 doses of the HPV-16/18/31/45 L1/AS04 vaccine, formulation 2 intramuscularly, into the deltoid of the non-dominant arm according to a 3-dose 0, 1, 6-month vaccination schedule.
HPV-TETRA E GroupHPV-16/18/31/45 L1 AS04 Formulation 5Female subjects who received 3 doses of the HPV-16/18/31/45 L1/AS04 vaccine, formulation 5 intramuscularly, into the deltoid of the non-dominant arm according to a 3-dose 0, 1, 6-month vaccination schedule.
Primary Outcome Measures
NameTimeMethod
Number of Seroconverted Subjects for Anti-Human Papillomavirus (Anti-HPV)-16 at Month 7At Month 7

Seroconversion was defined as the appearance of anti-HPV-16 antibodies \[i.e. antibody titer greater than or equal to (≥) the cut-off value\] in the serum of subjects seronegative before vaccination. The cut-off value was 8 enzyme-linked immunosorbent assay (ELISA) units per milliliter (EL.U/mL).

Number of Seroconverted Subjects for Anti-HPV-18 at Month 7At Month 7

Seroconversion was defined as the appearance of anti-HPV-18 antibodies (i.e. antibody titer ≥ cut-off value) in the serum of subjects seronegative before vaccination. The cut-off value was 7 EL.U/mL.

Anti-HPV-16 Antibody Titers Assessed by ELISA at Month 7At Month 7

Anti-HPV-16 antibody titers were presented as Geometric Mean Titers (GMT) and expressed in EL.U/mL.

Anti-HPV-18 Antibody Titers Assessed by ELISA at Month 7At Month 7

Anti-HPV-18 antibody titers were presented as Geometric Mean Titers and expressed in EL.U/mL.

Secondary Outcome Measures
NameTimeMethod
Number of Seroconverted Subjects for Anti-HPV-16 at Month 2At Month 2

Seroconversion was defined as the appearance of anti-HPV-16 antibodies (i.e. antibody titer ≥ cut-off value) in the serum of subjects seronegative before vaccination. The cut-off value was 8 EL.U/mL.

Number of Seroconverted Subjects for Anti-HPV-18 at Month 2At Month 2

Seroconversion was defined as the appearance of anti-HPV-18 antibodies (i.e. antibody titer ≥ cut-off value) in the serum of subjects seronegative before vaccination. The cut-off value was 7 EL.U/mL.

Anti-HPV-16 Antibody Titers Assessed by ELISA at Month 2At Month 2

Anti-HPV-16 antibody titers were presented as Geometric Mean Titers and expressed in EL.U/mL.

Anti-HPV-18 Antibody Titers Assessed by ELISA at Month 2At Month 2

Anti-HPV-18 antibody titers were presented as Geometric Mean Titers and expressed in EL.U/mL.

Number of Seroconverted Subjects for Anti-HPV-31 at Months 2 and 7At Month 2 and Month 7

Seroconversion was defined as the appearance of anti-HPV-31 antibodies (i.e. antibody titer ≥ cut-off value) in the serum of subjects seronegative before vaccination. The cut-off value was 59 EL.U/mL.

Number of Seroconverted Subjects for Anti-HPV-45 at Months 2 and 7At Month 2 and Month 7

Seroconversion was defined as the appearance of anti-HPV-45 antibodies (i.e. antibody titer ≥ cut-off value) in the serum of subjects seronegative before vaccination. The cut-off value was 59 EL.U/mL.

Anti-HPV-31 Antibody Titers Assessed by ELISA at Months 2 and 7At Month 2 and Month 7

Anti-HPV-31 antibody titers were presented as Geometric Mean Titers and expressed in EL.U/mL.

Anti-HPV-45 Antibody Titers Assessed by ELISA at Months 2 and 7At Month 2 and Month 7

Anti-HPV-45 antibody titers were presented as Geometric Mean Titers and expressed in EL.U/mL.

Number of Subjects With Any and Grade 3 Solicited Local SymptomsDuring the 7 day post-vaccination period following each dose and across doses

Assessed solicited local symptoms were pain, redness and swelling. Any = occurrence of any solicited local symptom regardless of their intensity grade. Grade 3 pain = significant pain at rest, that prevented normal every day activity. Grade 3 redness/swelling = redness/swelling above 50 millimeters (mm). All the reported local symptoms are considered related to the vaccination in the study.

Number of Subjects With Any, Grade 3 and Related Solicited General SymptomsDuring the 7 day post-vaccination period following each dose and across doses

Assessed solicited general symptoms were arthralgia, fatigue, fever, gastrointestinal symptoms, headache, myalgia, rash and urticaria. Any = occurrence of any solicited general symptom regardless of intensity grade or relationship to vaccination. Any Fever = axillary temperature greater than or equal to (≥) 37.5 degrees Celsius (°C). Grade 3 symptom = symptom that prevented normal activity. Grade 3 fever = fever greater than (\>) 39.0°C. Related = general symptom assessed by the investigator as causally related to the vaccination.

Number of Subjects With Any, Grade 3 and Related Unsolicited Adverse Events (AEs)During the 30-day post-vaccination period

An unsolicited adverse event (AE) was defined as any AE reported in addition to those solicited during the clinical study. Also, any "solicited" symptom with onset outside the specified period of follow-up for solicited symptoms was reported as an unsolicited adverse event. Any is defined as the occurrence of any unsolicited AE irrespective of its intensity grade and relationship to vaccination. Grade 3 unsolicited AE = an AE that prevented normal, everyday activities. Related AE = an AE assessed by the investigator as causally related to the study vaccination.

Number of Subjects With Any Serious Adverse Events (SAEs)From Day 0 to Month 7

SAEs assessed include medical occurrences that resulted in death, were life-threatening, required hospitalization or prolongation of hospitalization or resulted in disability/incapacity or were a congenital anomaly/birth defect in the offspring of a study subject. Any was defined as occurrence of any symptom regardless of intensity grade or relation to vaccination.

Number of Subjects With Any SAEs During the Extended Safety Follow-upFrom Day 0 to Month 12

SAEs assessed include medical occurrences that resulted in death, were life-threatening, required hospitalization or prolongation of hospitalization or resulted in disability/incapacity or were a congenital anomaly/birth defect in the offspring of a study subject. Any was defined as occurrence of any symptom regardless of intensity grade or relation to vaccination.

Number of Subjects Reporting Pregnancies and Outcomes of Reported PregnanciesFrom Day 0 to Month 12

Outcomes of reported pregnancies were: Healthy baby, Spontaneous abortion, Elective abortion.

Number of Subjects Reporting New Onset of Chronic Diseases (NOCDs)From Day 0 up to Month 12

NOCDs assessed include chronic diseases such as autoimmune disorders, diabetes, allergies also asthma and pathognomic signs/symptoms of these diseases.

Number of Subjects Reporting Medically Significant ConditionsFrom Day 0 up to Month 12

Medically significant conditions are AEs prompting emergency room or physician visits that were not related to common diseases or routine visits for physical examination or vaccination, or SAEs that were not related to common diseases.

Number of Subjects With Clinically Relevant Abnormalities in Biochemical and Haematological ParametersAt Month 2 and Month 7

The parameters assessed were both biochemical (alanine aminotransferase = ALAT, creatinine = CREA, blood urea nitrogen = BUN) and haematological \[basophils = BAS, eosinophils = EOS, red blood cells = RBC, hematocrit = HCT, hemoglobin = HGB, leukocytes (white blood cells) = WBC, lymphocytes = LYM, monocytes = MONO, neutrophils = NEU and platelets = PLA\]. Abnormal values of a parameter at Months 2 and 7 are defined as below and above the normal ranges, as compared to the baseline status of the same parameter.

Trial Locations

Locations (1)

GSK Investigational Site

🇧🇪

Wilrijk, Belgium

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