Effect of Fentanyl on Expression of Main Opioid Receptor (OPRM1) on Human Granulosa Cells During Ultrasound-guided Transvaginal Oocyte Retrieval.
试验速览
- 阶段
- 不适用
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- fentanyl
研究概览
简要总结
Opioids is known that produce not only analgesia but also hyperalgesia through activation of central glutaminergic system-GABA. At the same time, recently it was found that the main opioid receptor (OPRM1) is present on human granulosa cells and exogenous opiates and their antagonists can influence granulosa cell vascular endothelial growth factor (VEGF) production via OPRM1, causing ovarian hyperstimulation syndrome.
This study aims to investigate if a single exposure to opioids is enough to produce activation of stress mechanism during oocyte retrieval.
详细描述
The main opioid receptor (OPRM1) is present on human granulosa cells and exogenous opiates and their antagonists can influence granulosa cell vascular endothelial growth factor (VEGF) production via OPRM1, causing ovarian hyperstimulation syndrome.
This study aims to investigate if a single exposure to opioids is enough to produce activation of stress mechanism during ultrasound-guided oocyte retrieval. It will be measured the level of cortisone before and 15min after iv administration of fentanyl on blood and on oocyte fluid.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 32 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •American Physical Status I-III, BMI<30,
排除标准
- •Heart failure
- •hepatic failure, hepatitis,
- •drug abuse
- •receiving b blockers
- •receiving b agonists (even bronchodilators)
研究组 & 干预措施
Fentanyl citrate
Baseline blood sample and oocyte fluid will be collected under propofol anesthesia. Fifteen minutes after administration of 1γ/kgfentanyl, it will be collected again blood sample and oocyte fluid. Cortisone and fentanyl level will be measured in all samples.
干预措施: Fentanyl Citrate (Drug)
结局指标
主要结局
fentanyl
时间窗: 15 minutes
fentanyl on blood sample and oocyte fluid
次要结局
- cortisone(15 min)
研究者
Asouhidou Irene
Assisting Professor
George Papanicolaou Hospital