Effect of Fentanyl on Expression of Main Opioid Receptor (OPRM1) on Human Granulosa Cells During Ultrasound-guided Transvaginal Oocyte Retrieval.
Overview
- Phase
- Not Applicable
- Intervention
- Fentanyl Citrate
- Conditions
- Stress Reaction
- Sponsor
- George Papanicolaou Hospital
- Enrollment
- 30
- Locations
- 1
- Primary Endpoint
- fentanyl
- Last Updated
- 8 years ago
Overview
Brief Summary
Opioids is known that produce not only analgesia but also hyperalgesia through activation of central glutaminergic system-GABA. At the same time, recently it was found that the main opioid receptor (OPRM1) is present on human granulosa cells and exogenous opiates and their antagonists can influence granulosa cell vascular endothelial growth factor (VEGF) production via OPRM1, causing ovarian hyperstimulation syndrome.
This study aims to investigate if a single exposure to opioids is enough to produce activation of stress mechanism during oocyte retrieval.
Detailed Description
The main opioid receptor (OPRM1) is present on human granulosa cells and exogenous opiates and their antagonists can influence granulosa cell vascular endothelial growth factor (VEGF) production via OPRM1, causing ovarian hyperstimulation syndrome. This study aims to investigate if a single exposure to opioids is enough to produce activation of stress mechanism during ultrasound-guided oocyte retrieval. It will be measured the level of cortisone before and 15min after iv administration of fentanyl on blood and on oocyte fluid.
Investigators
Asouhidou Irene
Assisting Professor
George Papanicolaou Hospital
Eligibility Criteria
Inclusion Criteria
- •American Physical Status I-III, BMI\<30,
Exclusion Criteria
- •Heart failure
- •hepatic failure, hepatitis,
- •drug abuse
- •receiving b blockers
- •receiving b agonists (even bronchodilators)
Arms & Interventions
Fentanyl citrate
Baseline blood sample and oocyte fluid will be collected under propofol anesthesia. Fifteen minutes after administration of 1γ/kgfentanyl, it will be collected again blood sample and oocyte fluid. Cortisone and fentanyl level will be measured in all samples.
Intervention: Fentanyl Citrate
Outcomes
Primary Outcomes
fentanyl
Time Frame: 15 minutes
fentanyl on blood sample and oocyte fluid
Secondary Outcomes
- cortisone(15 min)