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Glucocorticoid-induced inhibition of IGF-I activity: exploration of underlying mechanisms.

Phase 1
Conditions
The catabolic effects induced by long-term glucocorticoid treatment.
Therapeutic area: Analytical, Diagnostic and Therapeutic Techniques and Equipment [E] - Therapeutic techniques [E02]
Registration Number
EUCTR2012-003504-12-DK
Lead Sponsor
Medical Research Laboratory, Clinical institute of Medicine, Aarhus University Hospital
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
ot Recruiting
Sex
Male
Target Recruitment
Not specified
Inclusion Criteria

Signed and dated informed consent
Healthy subjects
Sex: male
Age 20-30 years
BMI 19-26 kg/m2
normal HbA1c
Are the trial subjects under 18? no
Number of subjects for this age range:
F.1.2 Adults (18-64 years) yes
F.1.2.1 Number of subjects for this age range 20
F.1.3 Elderly (>=65 years) no
F.1.3.1 Number of subjects for this age range

Exclusion Criteria

Suspected or known allergy to the trial drug or similar medications.
Known current illness including diabetes mellitus, ischemic heart disease or cardiac arrhytmia.
Daily drug intake (excluding OCT preparations).
Known or previous mental illness.
Current participation or previous participation in experiments using ionizing radiation for a year prior to inclusion in this study.
participation in a larger X-ray examinations in trial period

Study & Design

Study Type
Interventional clinical trial of medicinal product
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Main Objective: The main purpose of the tríal is to advance our knowledge on the possible mechanism underlying the catabolic effects of long-term treatment with glucocorticoid. More specifically we are interested in the molecular mechanisms by which glucocorticoid inhibits growth.;Primary end point(s): GH and IGF-profiles of subjects before and after prednisolon-treatment. ;Timepoint(s) of evaluation of this end point: blood-test before (day 1) during (day 3) and at the end (day 5) of placebo/prednisolon treatment. ;Secondary Objective: To investigate the effect of glucocorticoid treatment on the activity of the circulating Insulin-like Growth Factor (IGF) system in healthy adults and intracellular in vitro and in vivo studies of IGF-I receptor phosphorylation and intracellular signaling in muscle and tissue biopsies. IGF levels in in serum vs. interstitiel fluid.
Secondary Outcome Measures
NameTimeMethod
Secondary end point(s): insulin sensitivity and intracellulair signaling of IGF-I under the influence/abscence of prednisolon;Timepoint(s) of evaluation of this end point: hyperinsulinemic euglycemic clamp and tissue biopsi on day 5.
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