Assessing Osteogenic Activity in Injectable Platelet Rich Fibrin Coated Implants Using Bone-specific Alkaline Phosphatase Biomarker An Observational Study

This observational study will aim to evaluate the osteogenic potential of dental implants coated with injectable Platelet-Rich Fibrin (i-PRF) by assessing levels of Bone-specific Alkaline Phosphatase (BALP), a biomarker indicative of bone formation activity.

A total of 28 systemically healthy patients, aged between 20 and 65 years and requiring implant placement, will be selected and randomly divided into two groups:

• Test Group (n=14): Will receive implants coated with i-PRF.
• Control Group (n=14): Will receive implants without any coating.

All implant procedures will be performed following standard surgical protocols. Peri-implant sulcular fluid (PISF) will be collected from each patient at 2, 4, and 8 weeks following implant placement. The levels of BALP in these samples will be analyzed using an enzyme-linked immunosorbent assay (ELISA) to compare osteogenic activity between the coated and uncoated implant groups.

The application of i-PRF, an autologous blood derivative rich in growth factors, is expected to enhance early osseointegration by promoting osteoblastic activity and bone formation. This study will aim to provide further insight into the clinical advantages of bioactive surface coatings in improving implant success and reducing healing time.

Statistical analysis will be performed using SPSS v21.0 to determine any significant differences in BALP levels across time intervals and between the two groups.