Rapid Measurements of Water Isotopes in Human Breath and Saliva for Doubly Labeled Water Analysis
试验速览
- 阶段
- 不适用
- 状态
- Enrolling By Invitation
- 入组人数
- 60
- 试验地点
- 2
- 主要终点
- Natural abundances of 2H:1H and 18O:16O in humans
研究概览
简要总结
This study proposes to demonstrate the feasibility of measuring 2H:1H and 18O:16O in saliva and breath by comparing the natural abundances of 2H:1H and 18O:16O in saliva and urine of un-dosed humans.
详细描述
The scientific premises for this line of research are 1) challenges related to costs, the complexity of sample collection and preparation, and the level of technical skills required for Isotope-ratio mass spectrometry (IRMS) analysis have limited the adoption of the doubly labeled water (DLW) method; and 2) the investigators preliminary data demonstrates that Off-Axis Integrated Cavity Output Spectroscopy (OA-ICOS) provides a valid, more rapid, and less technically challenging approach to isotopic analysis of human samples.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy adult men and women, aged 18-45 yrs
排除标准
- •Obesity (body mass index (BMI) > 30 kg/m2)
- •Pregnant or lactating women
- •Self-reported acute or chronic disease (diabetes, heart diseases, thyroid diseases)
结局指标
主要结局
Natural abundances of 2H:1H and 18O:16O in humans
时间窗: Baseline
Evaluation of the natural abundances of 2H:1H and 18O:16O in urine, saliva, and breath of humans.
次要结局
未报告次要终点