Project A4sc - An Atlas of Airways at a Single Cell Level

Not Applicable

Completed

• Conditions: Respiratory System
• Interventions: Procedure: Bronchoscopy

• Registration Number: NCT04204291
• Lead Sponsor: Centre Hospitalier Universitaire de Nice

Brief Summary

The increasing incidence of chronic respiratory diseases is a public health problem affecting hundreds of thousands of people around the world, including children. Directly exposed to atmospheric aerocontaminants (pollution, allergens), the respiratory tracts represent a complex ecosystem that involves different cells that develop complex interactions with the surrounding connective tissue but also with their rich immune environment and with the microbiota. Although a pathophysiological continuum is postulated between the nasal and bronchial airways in certain diseases, such as allergic diseases, we have identified broad gradients in gene expression between nasal and bronchial samples. This is why cellular variability throughout the respiratory tree needs to be studied in detail.

The sequencing of RNAs specifically present in a particular cell, and its comparison with neighboring cells, allows us to document precise cellular contributions and intercellular relationships. Our project will establish protocols to stabilize airway swabs by brushing and/or biopsy, under conditions that will then allow the analysis of gene expression profiles at the single cell level (single cell RNA sequencing).

The development of the "single cell" stabilisation and analysis protocol will first be carried out on primary respiratory epithelium cultures and then extended to respiratory specimens taken from healthy volunteers. Through sampling at several levels of the respiratory tree, variations in expression along the tracheobronchial axis will be fully documented. Finally, the interaction between the epithelial compartment and the immunological compartment will be studied by analyzing gene expression on a single cell in different physiopathological contexts.

Detailed Description

Not available

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study First Submitted: 2019-12-17
• Study First Submitted QC: 2019-12-17
• Study First Posted: 2019-12-18
• Study Start: 2020-06-23
• Primary Completion: 2021-06-25
• Study Completion: 2021-06-25
• Status Verified: 2024-02
• Last Update Submitted: 2024-02-05
• Last Update Posted: 2024-02-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 6

Inclusion Criteria

• age <40 years
• non-smoker
• absence of known acute or chronic disease
• absence of symptoms suggestive of progressive disease
• normal clinical examination

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Exclusion Criteria

• recent rhino-bronchial infection (<6 weeks)
• mental disability
• pregnant women (a urine test will be performed for all women of childbearing age)

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
All	Bronchoscopy	-

Primary Outcome Measures

Name	Time	Method
Number of samples usable	6 months

Trial Locations

Locations (1)

CHU de Nice; 🇫🇷 Nice, France