Influence of Dietary Fiber-rich Meals on Gene Expression and Postprandial Glucose and Lipid Response

Not Applicable

Completed

• Conditions: Hypoglycemia; Hyperglycemia
• Interventions: Other: Mixture of fiber; Other: Sugar beet fiber; Other: Rye bran; Other: Oat bran; Other: Spray-dried oat drink; Other: Control

• Registration Number: NCT01005342
• Lead Sponsor: Lund University

Brief Summary

The aim of this study is to

* Measure the effect on gene expression in leukocytes from a meal rich in oat bran

* Investigate the postprandial glucose, insulin and triglyceride responses after intake of meals containing fiber from different sources (oat, rye and sugar beet fiber) or a meal containing a mixture of these three fibers

Detailed Description

Dietary fiber has long been known to give beneficial health effects. Yet, the understanding of how fiber-rich meals regulate molecular events at a gene level is limited. Also, few studies have compared the effects of different fiber sources on postprandial responses and hardly any study the effects of fiber mixtures in the same meal, even though this is more similar to regular eating habits.

Healthy subjects will come to the study center after an overnight fast, to ingest breakfasts randomly enriched with different fiber. The meals contains either spray-dried oat drink, rye bran, sugar beet fiber a mixture of these three fibers, oat bran or no added fiber (control). All meals are adjusted to contain the same total amount of available carbohydrates and fat. Blood leukocytes for gene expression profiling were taken before and 2 h after consumption while blood samples for analysis of postprandial glucose, insulin and triglyceride levels were taken every 30 min during 3 h.

NuGO Affymetrix Human Genechip NuGO_Hs1a520180 are used for the microarray analysis and analysis is performed with linear mixed models and enrichment analysis to identify functional gene sets that responded to the specific oat bran effect.

Study Timeline

• Study Start: 2007-05
• Primary Completion: 2007-06
• Study Completion: 2007-06
• Status Verified: 2009-10
• Study First Submitted: 2009-10-29
• Study First Submitted QC: 2009-10-29
• Last Update Submitted: 2009-10-29
• Study First Posted: 2009-10-30
• Last Update Posted: 2009-10-30

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 18

Inclusion Criteria

• BMI 18-30

Exclusion Criteria

• pregnancy
• breastfeeding
• diabetes mellitus
• hepatitis B
• blood lipid lowering pharmaceuticals
• intolerance or allergy to cereals or sugar beet fiber

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: FACTORIAL

Arm && Interventions

Group	Intervention	Description
Mixture of fiber	Mixture of fiber	Single intake of a mixture of spray-dried oat drink, rye bran and sugar beet fiber
Sugar beet fiber	Sugar beet fiber	Single intake of sugar beet fiber
Rye bran	Rye bran	Single intake of rye bran
Oat bran	Oat bran	Single intake of oat bran
Spray-dried oat drink	Spray-dried oat drink	Single intake of spray-dried oat drink
Control	Control	Single intake of a meal with no added fiber

Primary Outcome Measures

Name	Time	Method
Changed gene expression profile by fiber-rich meals	2 h after meal intake

Secondary Outcome Measures

Name	Time	Method
Lowering of postprandial glucose by fiber-rich meals	0-180 min after meal intake

Trial Locations

Locations (1)

Lund University; 🇸🇪 Lund, Sweden