COMPARATIVE ANALYSIS OF THE MORPHO-MECHANICAL PROPERTIES OF RED BLOOD CELLS EXTRACTED FROM DIABETIC PATIENTS WITH AND WITHOUT MICROVASCULAR COMPLICATIONS
Trial Snapshot
- Phase
- Not Applicable
- Status
- Not yet recruiting
- Enrollment
- 48
- Primary Endpoint
- In 48 subjects with type 1 diabetes with and without microvascular complications, the average stiffness of red blood cells, measured using atomic force microscope and quantified using Young's modulus, will be measured.
Study Overview
Brief Summary
The aim of the study is to analyse the biomechanical stiffness of red blood cells extracted from subjects with type I diabetes with and without microvascular complications through a biophysical characterization of red blood cells using microscopy, spectroscopy and scattering measurements. 48 subjects with type 1 diabetes, 24 with and 24 without microvascular complications will be enrolled. From all subjects a single blood sample will be taken to perform the analysis of red blood cells morpho-mechanical properties.
Study Design
- Study Type
- Observational
- Observational Model
- Case Control
- Time Perspective
- Cross Sectional
Eligibility Criteria
- Ages
- 50 Years to 70 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Diagnosis of type 1 diabetes for at least 10 years
- •Stable metabolic control (HbA1c <7.5%) for at least 6 months
- •BMI between 18.5 and 30 Kg/m^2
- •Systolic blood pressure < 140 mmHg and diastolic blood pressure <90 mmHg
Exclusion Criteria
- •Blood pressure higher than 140/90 mmHg
- •Unstable metabolic control (HbA1c >7.5%)
- •BMI > 30 Kg/m^2 or < 18.5 Kg/m^2
- •Liver failure
- •Concomitant acute infections
- •Uncontrolled thyroid diseases
- •Pregnancy
Outcomes
Primary Outcomes
In 48 subjects with type 1 diabetes with and without microvascular complications, the average stiffness of red blood cells, measured using atomic force microscope and quantified using Young's modulus, will be measured.
Time Frame: 1 day
Young's modulus values are obtained using atomic force microscopy
Secondary Outcomes
No secondary outcomes reported