Real-time Amino acid Profiling

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 12

Inclusion Criteria

* Age *65 and *80
* BMI *20 and *32 kg/m2
* Non-smoking
* Healthy as assessed by the NIZO lifestyle and health questionnaire
(*Verklaring leefgewoonten en gezondheid*) and according to the judgment of the
study physician.
* Regular and normal Dutch eating habits as assessed by the NIZO lifestyle and
health questionnaire (3 main meals per day)
* Veins suitable for cannulation (blood sampling)
* Voluntary participation
* Having given written informed consent
* Willing to comply with study procedures
* Accept use of all encoded data, including publication, and the confidential
use and storage of all data for 15 years.
* Accept disclosure of the financial benefit of participation in the study to
the authorities concerned

Exclusion Criteria

* Participation in any clinical trial including blood sampling and/or
administration of substances up to 30 days before day 1 of this study
* Having a history of medical or surgical events that may significantly affect
the study outcome, including: Inflammatory bowel disease, hepatitis,
pancreatitis, ulcers, gastrointestinal or rectal bleeding; major
gastrointestinal tract surgery such as gastrectomy, gastroenterostomy, or bowel
resection; known or suspected gastrointestinal disorders, colon or GI tract
cancer
* Use of the following medication: glucose lowering drugs, insulin; medication
that may impact gastric emptying (e.g. gastric acid inhibitors or laxatives)
* Diagnosed with diabetes, being treated for high blood glucose or increased
fasting blood glucose (> 7 mmol/l in finger prick blood) as assessed during
screening visit
* For men: Hb <8,5 mmol/l as assessed during screening visit; for women: Hb
<7,5 mmol/l.
* Use of protein supplements
* Mental status that is incompatible with the proper conduct of the study
* A self-reported reported food allergy or sensitivity to dairy ingredients
* Alcohol consumption for men > 28 units/week and >4/day; for women: >21
units/week and >3/day
* Reported unexpected weight loss or weight gain of > 3 kg in the month prior
to pre-study screening, or intention to lose weight during the study period
* Reported slimming or medically prescribed diet
* Recent blood donation (<1 month prior to Day 01 of the study)
* Not willing to give up blood donation during the study
* Personnel of NIZO food research and FrieslandCampina, their partner and their
first and second degree relatives
* Not having a general practitioner
* Not willing to accept information-transfer concerning participation in the
study, or information regarding his or her health, like laboratory results and
eventual adverse events to and from his general practitioner

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Blood amino acid concentrations before and at 13 time points (up till 5 hours)<br /><br>after consumption of the dairy products. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Glucose- and insulin concentrations in blood before and at 13 time points (up<br /><br>till 5 hours) after consumption of the dairy products</p><br>