The Role of Congenital Mucosal Immune System in the Airways of Patients With Chronic Obstructive Pulmonary Disease
- Conditions
- Smoking, TobaccoCOPD Exacerbation
- Registration Number
- NCT04061122
- Lead Sponsor
- Medical University of Lodz
- Brief Summary
The purpose of the study is to explore the role of congenital mucosal immune system in the airways of patients with COPD including the impact of ILCs (Innate lymphoid cells) on metabolism of epithelial cells.During the research the following methods will be applied: measurement of Volatile Organic Compounds (VOCS), airway epithelial cytology and culture.
- Detailed Description
ILCs profiles, epithelium metabolism measured by VOCS and microbiome profiling (specific 16S rRNA-16S ribosomal RNA gene-targeted qPCR) will by determined in the airways of patients with COPD after the exacerbation. Microbiome profiling will be based on next generation sequencing (NGS) of the 16S rRNA encoding region.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 40
Not provided
- Neoplasm
- Diabetes
- Astma
- acute airway infection in last 2 weeks
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method percentage of nasal ILCs (Innate lymphoid cells) after COPD exacerbation through study completion, an average of 1 year The assessment of the nasal mucosa material after COPD exacerbation will include: percentage of ILC1, 2 and 3 cells (immunophenotyping and measurement by flow cytometry)
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Department of Internal Medicine, Asthma and Allergy, Barlicki University Hospital, Medical University of Lodz, Lodz, Poland
🇵🇱Lodz, Poland
Department of Internal Medicine, Asthma and Allergy, Barlicki University Hospital, Medical University of Lodz, Lodz, Poland🇵🇱Lodz, PolandPawel Majak, MD, PhDContactpawel.majak@umed.lodz.pl