The Role of Congenital Mucosal Immune System in the Airways of Patients With COPD
试验速览
- 阶段
- 不适用
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- percentage of nasal ILCs (Innate lymphoid cells) after COPD exacerbation
研究概览
简要总结
The purpose of the study is to explore the role of congenital mucosal immune system in the airways of patients with COPD including the impact of ILCs (Innate lymphoid cells) on metabolism of epithelial cells.During the research the following methods will be applied: measurement of Volatile Organic Compounds (VOCS), airway epithelial cytology and culture.
详细描述
ILCs profiles, epithelium metabolism measured by VOCS and microbiome profiling (specific 16S rRNA-16S ribosomal RNA gene-targeted qPCR) will by determined in the airways of patients with COPD after the exacerbation. Microbiome profiling will be based on next generation sequencing (NGS) of the 16S rRNA encoding region.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- 未提供
排除标准
- •acute airway infection in last 2 weeks
结局指标
主要结局
percentage of nasal ILCs (Innate lymphoid cells) after COPD exacerbation
时间窗: through study completion, an average of 1 year
The assessment of the nasal mucosa material after COPD exacerbation will include: percentage of ILC1, 2 and 3 cells (immunophenotyping and measurement by flow cytometry)
次要结局
未报告次要终点
研究者
Pawel Majak
Principal Investigator
Medical University of Lodz