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Fe Absorption in Mother and Child Pairs From Wheat Fortified With Iron With and Without Phenolic Containing Beverages

Not Applicable
Completed
Conditions
Anemia; Deficiency, Nutritional, With Poor Iron Absorption
Interventions
Other: Ferrous sulfate fortified bread with tea
Other: Ferrous fumarate fortified bread with tea
Other: ferrous sulfate fortified bread with water
Other: Ferrous fumarate fortified bread with water
Registration Number
NCT02437955
Lead Sponsor
Swiss Federal Institute of Technology
Brief Summary

In Senegal, iron deficiency affects 39% of and 82% of women and children between 12 and 50 months indicating that iron deficiency is a major health problem. The government of Senegal has implemented a flour fortification program including iron and folate. Iron is a difficult mineral to add to foods efficiently due to its organoleptic properties and typical low bioavailability in man. The aim of this study is to determine iron absorption from fortified wheat flour consumed with a phenolic containing beverage in women and child pairs. The fortificants used will be Ferrous Fumarate and Ferrous Sulfate, and the effect of absorption inhibitors on the bioavailability from iron compounds not readily bioavailable is poorly investigated.

The bioavailability of the different iron compounds will be determined using stable iron isotopes. Sixteen pairs of women and children (n=34, children between 3-6 years, women between 18-45y) will be selected for participation in the study. After a screening, each women and child will receive 4 test meals consisting of a bread roll fortified stable isotopes with and without the tisane beverage. The first two test meals will be consumed on consecutive days. Two weeks after the second test meal a blood sample will be taken from each women and child before the third meal administration. After the forth test meal administration, and again two weeks later, the last blood sampling will take place and the study will be conducted for the subjects (duration 30 days). The samples will be sent to Zurich on dry ice for analysis for the following parameters: isotopic composition, H pylori infection, B vitamin status, Vitamin A status. In all samples, hemoglobin and iron status as well as inflammation status (CRP) will be determined.

Detailed Description

In this study each subject will act as his own control and receive all interventions. Iron absorption data from the different test meals will be therefore compared assessing within and subject effects (main outcomes). Secondary outcomes will also investigate between subject effects and co variates of iron absorption.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
34
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
FESO4+TFerrous sulfate fortified bread with teaFerrous sulfate fortified bread with tea
FeFu+TFerrous fumarate fortified bread with teaFerrous fumarate fortified bread with tea
FESO4+Wferrous sulfate fortified bread with waterFerrous sulfate fortified bread with water
FeFu+WFerrous fumarate fortified bread with waterFerrous fumarate fortified bread with water
Primary Outcome Measures
NameTimeMethod
Iron absorption measured as iron incorporation of stable isotopic labels in red blood cells 14 days after administrationUp to 8 Months after study start

The effect of the consumption of tea (phenolic containing beverages) will be assessed the two iron fortification compounds. The interaction between beverage and compound on iron absorption will be tested. The analysis will be separately conducted for mothers and children.

Secondary Outcome Measures
NameTimeMethod
Correlation between iron absorption in mother and child pairsUp to 8 Months after study start

The correlation of iron absorption from the different test meals between in mother and child pairs will be assessed. A variable will be defined which will consist of the difference in iron absorption between each mother and child, within one mother child pair cluster. Determinants of this variable will be explored by linear regression, such as iron status, parity, age, gender, anthropometry, iron status, vitamin status (A, B2, B12, folate) h. pylori test and hepcidin.

Trial Locations

Locations (1)

Université Cheikh Anta Diop

🇸🇳

Dakar, Senegal

Université Cheikh Anta Diop
🇸🇳Dakar, Senegal
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