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Cryopreserved Human Umbilical Cord (TTAX01) for Late Stage, Complex Non-healing Diabetic Foot Ulcers (AMBULATE DFU)

Phase 3
Completed
Conditions
Non-healing Wound
Non-healing Diabetic Foot Ulcer
Diabetic Foot Infection
Interventions
Biological: TTAX01
Other: Standard Care
Registration Number
NCT04176120
Lead Sponsor
Tissue Tech Inc.
Brief Summary

It is hypothesized that application at 4-week or greater intervals of the human placental umbilical cord tissue TTAX01 to the surface of a well debrided, complex diabetic foot ulcer (DFU) will, with concomitant management of infection, will result in a higher rate of wounds showing complete healing within 26 weeks of initiating therapy, compared with standard care alone. This confirmatory Phase 3 study examines a population of diabetic foot ulcer patients having adequate perfusion, with or without neuropathy, and a high suspicion of associated osteomyelitis in a complex, high grade wound.

Detailed Description

This trial is designed as a confirmatory study of the benefits and risks of TTAX01 when used in the treatment of Wagner Grades 3 and 4 DFU. Experience with the use of a cryopreserved umbilical cord (UC) product in treating such wounds, both prior to this Investigational New Drug application (IND) and under this IND, has indicated that a frequency of application of no shorter than every 4 weeks is associated with better than expected outcomes. Although treatment cannot be blinded, a "standard care only" arm is included to control for the benefits of aggressive baseline debridement combined with aggressive (6 weeks systemic) antibiotics. Current treatment guidelines indicate that aggressive debridement plus 1-2 weeks of antibiotics, or, minor debridement plus 6 weeks of antibiotics, would produce equivalent outcomes, although the evidence is not strong. By utilizing both maximum debridement and maximum antimicrobial therapy, the standard care described in this protocol may result in healing rates somewhat superior to current standard practice.

The design of this confirmatory study is matched to the design of the Phase 2 efficacy study TTCRNE-1501, with the exception of extending the primary endpoint from 16 to 26 weeks, and utilizing a proportional risk analysis rather than a landmark analysis. This design consideration is based on analysis of previous studies, and a desire to fold data from every visit into the primary analysis, rather than generating an excessive number of secondary endpoints.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
220
Inclusion Criteria
  • The subject has signed the informed consent form
  • The subject is male or female, at least 18 years of age inclusive at the date of Screening
  • The subject has confirmed diagnosis of Type I or Type II diabetes
  • The subject's index ulcer is located on the plantar surface, inter digital, heel, lateral or medial surface of the foot
  • The subject has an index ulcer with visible margins having an area ≤ 12.0 cm2 when measured by the electronic measuring device at Screening
  • The subject's index ulcer extends beyond the dermis, into subcutaneous tissue with evidence of exposed bone, tendon, muscle and/or joint capsule
  • The subject presents with history, signs or symptoms leading to a clinical suspicion of osteomyelitis in the opinion of the Investigator supported by positive Probe to Bone (PTB) and any of the following: radiographic (X-ray, Magnetic Resonance Imaging (MRI), or bone scan) or evidence of bone necrosis
  • The subject has an ABI ≥ 0.7 to ≤ 1.3 or TcPO2 ≥ 40 mmHg on the dorsum of the affected foot, or Great Toe Pressure ≥ 50 mmHg
  • The subject is under the care of a physician for the management of Diabetes Mellitus
  • The subject is willing to return for all mandatory visits as defined in the protocol
  • The subject is willing to follow the instructions of the trial Investigator
Exclusion Criteria
  • The subject's index ulcer is primarily located on the dorsal surface of the foot
  • The subject's index ulcer can be addressed by primary closure through the completion of the initial or staged surgical procedure
  • The subject has a contralateral major amputation of the lower extremity
  • The subject has a glycated hemoglobin A1c (HbA1c) level of > 12%
  • The subject has been on oral steroid use of > 7.5 mg daily for greater than seven (7) consecutive days in 30 days before Screening
  • The subject has been on parenteral corticosteroids, or any cytotoxic agents for seven consecutive days in the period of 30 days before Screening
  • The subject is currently taking the type 2 diabetes medicine canagliflozin (Invokana™, Invokamet™, Invokamet XR™)
  • The subject has malignancy or a history of cancer, other than non-melanoma skin cancer, in five years before Screening
  • The subject is pregnant
  • The subject is a nursing mother
  • The subject is a woman of child-bearing potential who is unwilling to avoid pregnancy or use an appropriate form of birth control (adequate birth control methods are defined as: topical, oral, implantable, or injectable contraceptives; spermicide in conjunction with a barrier such as a condom or diaphragm; intrauterine device (IUD); or surgical sterilization of partner).
  • The subject is unable to sustain off-loading as defined by the protocol
  • The subject has an allergy to primary or secondary dressing materials used in this trial
  • The subject has an allergy to glycerol
  • The subject's index ulcer is over an acute Charcot deformity
  • The subject has had previous use of NEOX®, CLARIX®, or TTAX01 applied to the index ulcer
  • Per Investigator's discretion the subject is not appropriate for inclusion in the trial, e.g., undergoing surgical treatments listed in the protocol or the subject currently has sepsis, i.e., life-threatening organ dysfunction caused by a dysregulated host response to infection

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
TTAX01TTAX01TTAX01 plus standard care
TTAX01Standard CareTTAX01 plus standard care
ControlStandard CareStandard Care alone
Primary Outcome Measures
NameTimeMethod
Complete wound healingover 26 weeks

Time from baseline to initial observation of healing, where healing has been confirmed at two consecutive visits each two weeks apart.

Secondary Outcome Measures
NameTimeMethod
Complete wound healingby Week 50

Proportion of wound healed

Patient-reported mobilityBaseline to End of Study (approximately 50 weeks)

compare total score on PROMIS Health Organization Neuro-QoL 'lower extremity function'; scale from 1-5; higher scores indicate better self-reported health

Wound durabilityby Week 50

Proportion of wounds that re-ulcerate

Limb preservationby Week 50

proportion of minor and major amputations following the initial procedure

Trial Locations

Locations (16)

Carilion Clinic

🇺🇸

Roanoke, Virginia, United States

Center for Clinical Research, Inc

🇺🇸

San Francisco, California, United States

Vanderbilt University Medical Center

🇺🇸

Nashville, Tennessee, United States

Bio-X-Cell Research

🇺🇸

San Antonio, Texas, United States

University of Miami

🇺🇸

Miami, Florida, United States

Paley Orthopedic & Spine Institute

🇺🇸

West Palm Beach, Florida, United States

UCLA Olive View

🇺🇸

Sylmar, California, United States

Rosalind Franklin University

🇺🇸

North Chicago, Illinois, United States

Mount Sinai West

🇺🇸

New York, New York, United States

University Hospitals Richmond Medical Center

🇺🇸

Richmond Heights, Ohio, United States

Futuro Clinical Trials, LLC

🇺🇸

McAllen, Texas, United States

Foot & Ankle Institute

🇺🇸

Saint George, Utah, United States

JPS

🇺🇸

Fort Worth, Texas, United States

University of North Carolina

🇺🇸

Chapel Hill, North Carolina, United States

Ochsner Medical Center

🇺🇸

New Orleans, Louisiana, United States

Oregon Health & Science University

🇺🇸

Portland, Oregon, United States

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