Effect of Non-surgical Periodontal Therapy on Leptin and Visfatin Expression in Gingival Tissues of Chronic Periodontitis With and Without Type 2 Diabetes Mellitus: A Study Utilizing ELISA and Real Time PCR
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Cairo University
- Enrollment
- 50
- Locations
- 1
- Primary Endpoint
- visfatin levels in gingival tissues
Study Overview
Brief Summary
- Are adipokines involved in pathogenesis of periodontal disease in diabetic patients?
- Does their level change after periodontal therapy?
Detailed Description
Patients with chronic periodontitis only and patients with chronic periodontitis and diabetes were evaluated for levels of leptin and visfatin in the gingival tissues before and after non-surgical periodontal therapy.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Diagnostic
- Masking
- Single (Outcomes Assessor)
Eligibility Criteria
- Ages
- 35 Years to 54 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •diabetic patients having diabetes mellitus ≥5 years prior to the study.
- •chronic periodontitis with clinical attachment loss > 5 mm.
- •otherwise systemically healthy subjects.
Exclusion Criteria
- •any known systemic diseases other than diabetes.
- •pregnancy or lactation.
- •receiving periodontal treatment in the last 6 months.
- •taking any drugs or medications other than anti-diabetic drugs.
Outcomes
Primary Outcomes
visfatin levels in gingival tissues
Time Frame: 3 months
estimation of protein and gene expression of visfatin in patients with chronic periodontitis only and diabetic patients with periodontitis before and after non-surgical periodontal therapy..
leptin levels in gingival tissue
Time Frame: 3 months
estimation of protein and gene expression of leptin in patients with chronic periodontitis only and diabetic patients with periodontitis before and after non-surgical periodontal therapy..
Secondary Outcomes
- pocket depth(3 months)
- clinical attachment level(3 months)
Investigators
Noha Ayman Ghallab
Associate Professor of periodontology, faculty of oral and dental medicine
Cairo University