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Effects of Hypoxic-hyperoxic Preconditioning in Cardio-surgical Patients

Not Applicable
Completed
Conditions
Myocardial Reperfusion Injury
Coronary Artery Disease
Hypoxia
Hyperoxia
Interventions
Procedure: Hypoxic-hyperoxic preconditioning
Procedure: Placebo
Registration Number
NCT05354648
Lead Sponsor
Federal Research Clinical Center of Federal Medical & Biological Agency, Russia
Brief Summary

Coronary artery bypass grafting (CABG) with cardiopulmonary bypass is a common surgical therapy for patients suffering from coronary artery diseases. The heart is subjected to a long period of ischemia due to the occlusion of the aorta. The heavy burden of myocardial ischemia-reperfusion injury (IRI) thus induces cardiomyocyte death, which can paradoxically reduce the beneficial effect of CABG. Preconditioning by moderate hypoxia or hyperoxia serves as an effective drug-free method to increase the organism's resistance to negative effects, including IRI.

Detailed Description

It has been firmly established that the diminished oxygen delivery to the tissues in response to hypoxia is countered by a combination of the increased regional blood flow and the enhanced functional capillary density in the microcirculation. In experimental studies, exposure to hyperoxia for a limited time before ischemia induces a low-grade systemic oxidative stress evokes a preconditioning-like effect on the myocardium and reduces the infarction area by 20%, and the number of arrhythmias after ischemia-reperfusion. One hundred twenty patients were randomly assigned into two equal groups: hypoxic-hyperoxic preconditioning before the surgery (HHP group) and the control group (without preconditioning). Safety control of the preconditioning procedure included ECG monitoring, invasive blood pressure, cardiac output, pulse oximetry, capnography, cerebral oximetry, measurement of anaerobic threshold; acid-base status and metabolic state of arterial and venous blood were assessed once every 10 min during the preconditioning procedure; oxygen balance parameters were calculated. Seventy-two hours before the surgery, an anaerobic threshold was determined to establish a safe oxygen concentration in the respiratory gas mixture during the hypoxic preconditioning phase from 10 to 14%, followed by 75-80% oxygen concentration during the hyperoxic phase.

The hypoxic-hyperoxic preconditioning with individual parameters selection based on the anaerobic threshold in patients with coronary artery diseases before the main stage of cardiac surgery with a cardiopulmonary bypass should reduce the duration of mechanical ventilation, catecholamine support, and frequency of perioperative complications.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
120
Inclusion Criteria
  • the need for coronary artery bypass grafting (CABG)
Exclusion Criteria
  • age over 75 years
  • emergency surgery
  • diabetes mellitus
  • exacerbation of a chronic disease 1 week before surgery
  • any oncological disease at the time of the examination

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Hypoxic-hyperoxic preconditioning (HHP)Hypoxic-hyperoxic preconditioningHHP was carried out as follows: breathing with a hypoxic gas mixture for 10 min with the development of hypoxemia, then breathing with a hyperoxic gas mixture for 30 minutes, and at the last stage, a period of breathing with atmospheric air until the cardio-pulmonary bypass is connected. The anaerobic threshold was determined 72 hours before surgery to establish a safe oxygen concentration in the respiratory gas mixture during the hypoxic phase of preconditioning.
ControlPlaceboThe anaerobic threshold was determined, however, patients in the control group were not preconditioned. Mechanical ventilation was carried out with individual settings maintaining the target values of PaO2 and PaCO2 (80 - 120 mm Hg and 35 - 45 mm Hg, respectively), until the cardio-pulmonary bypass was connected.
Primary Outcome Measures
NameTimeMethod
Number of Participants with complications60 days

Any type of complications in postoperative period

Mechanical ventilation60 days

Mechanical ventilation time

Rate of spontaneous sinus rhythm recovery14 days

Spontaneous sinus rhythm recovery after surgery

Secondary Outcome Measures
NameTimeMethod
ADMA dymanics 224 hours after surgery

ADMA concentrations

Catecholamine support60 days

Catecholamine support time

Troponin T12 hours

Troponin T concentration

NOx total1 day before surgery

NOx total concentrations

Endothelin-11 day before surgery

Endothelin-1 concentrations

Endothelin-1 dynamics 224 hours after surgery

Endothelin-1 concentrations

NOx total dynamics 224 hours after surgery

NOx total concentrations

Asymmetric dimethylarginine (ADMA)1 day before surgery

ADMA concentrations

Endothelin-1 dynamics 1at the end of surgery

Endothelin-1 concentrations

NOx total dynamics 1at the end of surgery

NOx total concentrations

ADMA dymanics 1at the end of surgery

ADMA concentrations

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