Is microvascular dysfunction an early phenomenon in the development of skeletal muscle insulin resistance? A dietary intervention study in healthy me

Completed

Conditions: Obesity
overweight/fatness
10018424

Registration Number: NL-OMON44864

Lead Sponsor: Vrije Universiteit Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 15

Inclusion Criteria

1. Age 18*30 years
2. Male gender
3. Caucasian
4. BMI 20*25 kg/m2
5. Normal insulin sensitivity
6. Normoglycemia at time of screening
7. Normal diet pattern
8. Stable body weight during 6 months before enrolment in the study (<3% shifts)

Exclusion Criteria

1. Presence of any relevant disease
2. Use of any relevant medication
3. First-degree relative with type 2 diabetes
4. Smoking
5. Shift work
6. A history of chronic glucocorticoids use or GC use < 3months
7. Excessive sport activities

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1. Metabolic insulin sensitivity: rate of disappaerance (Rd) en endogenous<br /><br>glucose production (EGP)<br /><br>2. Vascular insuline senitivityt: changes in microvascular blood volume (MBV) </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>1. Microvascular vasoreactivity in vitro with and without PVAT (myograph<br /><br>technique)<br /><br>2. Insulin mediated protein expression and activation in microvessels, adipose<br /><br>and skeletal muscle tissue<br /><br>3. Gen expression of insulin signaling genes in endothelial cells<br /><br>4. PVAT morphology/characteristics, including adipocyte size, celltypes and<br /><br>expression of adipokines<br /><br>5. Plasma insulin, glucose, lipid spectrum, inflammatory parameters<br /><br>6. Physical activity (by means of accelerometry)<br /><br>7. Gut microbiota composition (fecal analysis)</p><br>