Study of Brain Derived Neurotrophic Factor (BDNF) Pathway Biomarkers in the Cerebrospinal Fluid in Patients With Huntington's Disease
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 135
- 试验地点
- 1
- 主要终点
- BDNF(csf) in HD subjects compared to age-matched control subjects (+/- 5 years)
研究概览
简要总结
Huntington disease (HD, 1.3/10 000) is an autosomal dominant disease due to an abnormal expansion of CAG triplets in HTT gene.
Several pathophysiological mechanisms have been evoked, including an alteration of the signaling pathway of the Brain Derived Neurotrophic Factor (BDNF), a neurotrophic factor involved in the survival of neurons (striatal and hippocampal) and synaptic plasticity. BDNF is synthesized at the level of cortical neurons and transported, through the axonal transport in which the Htt is involved, to the nerve endings; it's then secreted in response to excitatory synaptic activity, especially at the level of glutamatergic synapses. Besides, at the postsynaptic level it binds with great specificity to TrkB receptors (tropomyosin-related kinase receptors B) with a neuroprotective effect on dendritic and axonal growth and an increase in synaptic plasticity, especially at the level of the striatum and the hippocampus.
BDNF is decreased in the brain of animal models, as well as in patients with HD; the alteration of this pathway would occur in the early stages of the disease.
In the context of concomitant multiple treatments, the BNDF pathway may be one of the therapeutic targets of HD.
Moreover, in HD it remains essential to detect biological markers representative of the different pathogenic pathways that can be tested in vivo in humans to confirm the hypotheses developed at the level of basic research; these biomarkers could subsequently become biomarkers of disease progression and/or biomarkers of therapeutic efficacy of potential targeted treatments.
Therefore, this study aims to characterize potential biomarkers of the BNDF pathway in plasma and CSF in subjects with HD and to confirm the importance of this pathogenic mechanism in vivo in humans.
详细描述
- Design: Multicentre prospective case-control study. Centres: University Hospital of Montpellier, France; University Hospital of Bordeaux, France; University Hospital of Nimes, France; University Hospital of Poitiers, France.
- Main objective: To evaluate BDNF in cerebrospinal fluid as a potential marker of the BDNF-TrkB signaling pathway in vivo in HD patients at a symptomatic stage.
- Secondary objectives: i) Evaluate plasma BDNF in subjects with HD; ii) Study the correlation between BDNF in CSF and BDNF in plasma; iii) Study the correlation between markers of the BDNF pathway and clinical severity, multimodal brain MRI parameters, and relevant markers of evolution of HD; iv) Confirm the increase of Tau and NFL (Neurofilament Light Chain) markers in plasma and in CSF, as markers of neuronal degeneration, in subjects with HD ; v) Test the TrkB assay in the CSF of patients with HD
- Inclusion Criteria. General inclusion criteria: age ≥ 18 years old; national health insurance cover. Patient inclusion criteria: genetically confirmed Huntington's disease diagnosis (≥ 35 CAG repeat in HTT gene exon 1); written informed consent; patient agreement for LP, if requested. Control inclusion criteria: previous LP for medical reason; agreement for inclusion in a biobank for research purposes.
- Exclusion Criteria. General exclusion criteria: subject protected by law, under curatorship or guardianship. Patients exclusion criteria: too severe HD, according to the clinician's judgment, possibly making difficult to perform cognitive evaluation or MRI; contraindications to brain MRI; contraindications to LP; inability to give informed consent. Control exclusion criteria: presence of a neurodegenerative of inflammatory central nervous system disease.
- Inclusion period: 48 months
- Duration of participation for each patient: 123 days maximum
- Total research duration: 64 months
- Plan of the study. Patients group: in 90 patients with HD, the investigators will perform: a collection of the main anamnestic and clinical data; a blood test for the determination of plasmatic BDNF, Tau and NFL and the genotyping of the Val66Met polymorphism of the BDNF gene; multimodal brain MRI with volumetry, diffusion tensor, functional MRI of rest; a measurement of the severity of Huntington's disease and Total Functional Capacity scales; neuropsychological tests (SDMT, STROOP test, Trail Making Test (TMT) A and B, digit span). In a subgroup of 45 patients, the investigators will also perform a lumbar puncture for the determination of BDNF, Tau, NFL and TrkB in CSF. Control Group: 45 controls will be selected from the samples present in the existing Biobank with CSF and plasma samples available in Montpellier, France. MRI data will be centralized and processed by the Imaging Institute I2FH Montpellier University Hospital.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •General inclusion criteria:
- •age ≥ 18 years-old
- •national health insurance cover
- •Patients inclusion criteria:
- •genetically confirmed Huntington's disease diagnosis (≥ 35 CAG repeat in HTT gene exon 1)
- •written informed consent
- •only for patients "with lumbar puncture (LP)": patient agreement for LP
- •Control inclusion criteria:
- •anterior LP for medical reason with consent for biobank "Neuro" with following samples present in this biobank : 2 mL blood + 0.5 mL plasma + 0.5 mL cerebrospinal fluid
- •information and non-opposition for the finality of this biobank
- •paired by age with a patient (+/- 5 years difference)
排除标准
- •General exclusion criteria:
- •protected by law
- •Patients exclusion criteria:
- •Huntington's disease stage too Evolved that may interfere with cognitive evaluations or MRI
- •contraindications to brain MRI
- •only for patients "with LP": contraindications to LP
- •incapacity to give informed consent
- •Control exclusion criteria:
- •neurodegenerative of inflammatory central nervous system pathology
研究组 & 干预措施
Patient with LP
Huntington's disease patients who agreed to have LP
干预措施: Brain MRI (Procedure)
Patient with LP
Huntington's disease patients who agreed to have LP
干预措施: Lumbar Punction (Procedure)
Patient with LP
Huntington's disease patients who agreed to have LP
干预措施: Blood sample (Genetic)
Patient with LP
Huntington's disease patients who agreed to have LP
干预措施: Cognitive evaluation (Other)
Patient without LP
Huntington's disease patient with contraindication to LP or refusal to have LP
干预措施: Brain MRI (Procedure)
Patient without LP
Huntington's disease patient with contraindication to LP or refusal to have LP
干预措施: Blood sample (Genetic)
Patient without LP
Huntington's disease patient with contraindication to LP or refusal to have LP
干预措施: Cognitive evaluation (Other)
Control Group
Retrospective study with biologic samples of patients without Huntington's disease
结局指标
主要结局
BDNF(csf) in HD subjects compared to age-matched control subjects (+/- 5 years)
时间窗: Inclusion
centralized ELISA assay with Simoa - Quanterix kit technology at the Laboratory of Clinical Proteomic Biochemistry of Montpellier, France.
次要结局
- Correlation between BDNF in CSF and BDNF in plasma(Inclusion)
- Correlation between BDNF and disease parameters(Inclusion)
- Total Tau and NFL levels in plasma and CSF in HD subjects vs control subjects(Inclusion)
- TrkBcsf level in subjects with HD vs control subjects(Inclusion)
- plasmatic BDNF in HD subjects vs controls(Inclusion)