Impact of Muscle Temperature on Muscle Growth and Breakdown: Cooling During Resistance Training
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 12
- 试验地点
- 2
- 主要终点
- RT-qPCR for Myocyte Enhancer Factor 2A (MEF2a)
研究概览
简要总结
The purpose of this study is to examine the effect of human skeletal muscle temperature during resistance exercise on myogenic and proteolytic signaling. Subjects will perform bilateral resistance exercise bouts at an intensity that should stimulate a muscle growth response in the Vastus Lateralis. During the resistance exercise, the subjects will receive a cold (10°C) intervention on the experimental limb, while receiving a neutral temperature intervention (22°C) on the control limb.
详细描述
A total of 2 visits to the laboratory will be required following the signing of informed consent. All visits will take place at the University of Nebraska Exercise Physiology Laboratory.
Initial Visit (~1.5-2 hours):
Height and weight will be measured using a medical scale. Body fat will be assessed with hydrostatic weighing using an electronic load cell based system (Exertech, Dresbach, MN) correcting for residual lung volume, or Bioelectrical Impedance Analysis body composition analyzer (InBodyUSA, Cerritos, CA) if needed.
Subjects will then perform a 12 Repetition (12RM) Max Test for bilateral seated leg press and seated leg extension using the following protocol on isotonic exercise machines at a weight designed to create failure between 8 and 12 repetitions. Following a short warm-up on a cycle ergometer, the subjects proceed with a bilateral leg press warm-up consisting of one set of ten repetitions at a self-determined light load, then one set of 5 repetition at a self-determined moderate load, and one set of 1 repetition at a predicted 12 rep maximum as predicted in relation to their Fat Free Mass. During the warm-up subjects will be coached on form, and observed for their ability to complete the repetition max test. Weight for the test will be altered to suit the subject's ability to complete approximately 12 repetitions. Once the desired weight is determined, subjects will be asked to complete as many repetitions as possible until the subject can no longer complete a repetition using a full range of motion. An identical testing protocol is then performed for the leg extension. This data will be used to estimate a 12RM workload, that would be appropriate for hypertrophic growth using the Watham equation .
Experimental Visit (~6.5-8 hours):
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 19 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Any male or female who is 19-45 of age and is considered
- •low risk for physical activity based on the American College of Sports Medicine (ACSM) guidelines
- •recreationally active during the 6 weeks prior to participation by meeting the ACSM minimum activity guidelines
排除标准
- •Any person outside of the age range of 19-45
- •not considered 'low risk' on the ACSM Risk Stratification Form
- •not meeting the exercise requirements for the preceding 6 weeks will be excluded.
- •self-report of a history of joint or muscular injury that would prevent them from safely completing the required activities
- •self-report of taking prescription drugs or supplements that may interfere with this research
- •self-report allergy to Xylocaine
- •breast feeding
- •self-report sensitivity to heat or cold
结局指标
主要结局
RT-qPCR for Myocyte Enhancer Factor 2A (MEF2a)
时间窗: 4-hours post Exercise
RT-qPCR for Myocyte Enhancer Factor 2A (MEF2a) \[mRNA response of MEF2a\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Growth/Differentiation Factor 8 (MSTN)
时间窗: 4-hours post Exercise
RT-qPCR for Growth/Differentiation Factor 8 (MSTN) \[mRNA response of MSTN\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Forkhead Box O3 (FOXO3a)
时间窗: 4-hours post Exercise
RT-qPCR for Forkhead Box O3 (FOXO3a) \[mRNA response FOXO3a\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Tripartite Motif Containing 63 (TRIM63)
时间窗: 4-hours post Exercise
RT-qPCR for Tripartite Motif Containing 63 (TRIM63) \[mRNA response of TRIM63\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Myogenic Factor 5 (MYF5)
时间窗: 4-hours post Exercise
RT-qPCR for Myogenic Factor 5 (MYF5) \[mRNA response of MYF5\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Myogenic Factor 6 (MYF6)
时间窗: 4-hours post Exercise
RT-qPCR for Myogenic Factor 6 (MYF6) \[mRNA response of MYF6\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Myogenic Differentiation 1 (MYOD1)
时间窗: 4-hours post Exercise
RT-qPCR for Myogenic Differentiation 1 (MYOD1) \[mRNA response of MYOD1\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Myogenin (MYOG)
时间窗: 4-hours post Exercise
RT-qPCR for Myogenin (MYOG) \[mRNA response of MYOG\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Ribosomal Protein S3 (RPS3)
时间窗: 4-hours post Exercise
RT-qPCR for Ribosomal Protein S3 (RPS3) \[mRNA response of RPS3\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
RT-qPCR for Ribosomal Protein L3 Like (RPL3L)
时间窗: 4-hours post Exercise
RT-qPCR for Ribosomal Protein L3 Like (RPL3L) \[mRNA response of RPL3L\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.
次要结局
- Muscle Temperature(4-hours post Exercise)
- Skin Temperature(4-hours post Exercise)