WGS of NTM From Sputum of NTM Lung Disease Patients and From Their Environments
- Conditions
- Nontuberculous Mycobacterium InfectionWhole Genome Sequencing
- Registration Number
- NCT03532438
- Lead Sponsor
- Seoul National University Hospital
- Brief Summary
The purpose of the study is to identify the genetic closeness of NTM derived from patients living together and from their surrounding environment and to trace the temporal relationship between them.
- Detailed Description
After NTM isolation is done by using culture, from patients' sputum/bronchoscopic samples and their environmental samples, whole genome sequencing (WGS) will be conducted for all NTM isolates. Phylogenetic analysis will be performed based on single nucleotide polymorphism (SNP) on each NTM.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 6
- The adult over 18 years of age
- The patient who have respiratory symptoms consistent with NTM, such as a chronic cough, sputum, and hemoptysis, who have NTM-positive findings on CT, and who have NTM identification in two or more sputum cultures or in one or more bronchoscopic/tissue culture. (2007 ATS/IDSA guideline)
- Pairs of patients living in the same household since diagnosis on NTM lung disease
- Consent to a collection of environmental samples from the patient's residence.
- If the patient does not agree or withdraw.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Genetic similarity of NTM(Non-Tuberculous Mycobacterium) Baseline Concordance of Single Nucleotide Polymorphisms (SNP) from NTMs derived from patients and their environments
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Division of Pulmonology and Critical Care Medicine, Department of Internal Medicine and Lung Institute of Medical Research Center, Seoul National University College of Medicine
🇰🇷Seoul, Korea, Republic of