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Effect of Soluble Corn Fiber Supplementation for 1 Year on Bone Metabolism in Adolescents

Phase 2
Completed
Conditions
Osteoporosis
Interventions
Combination Product: Soluble Corn Fiber (SCF) + Calcium
Combination Product: Placebo + calcium
Dietary Supplement: Soluble Corn Fiber (SCF) without Calcium
Dietary Supplement: Placebo
Registration Number
NCT02916862
Lead Sponsor
Florida International University
Brief Summary

The primary aim of this proposal is to determine the effects of soluble corn fiber (SCF) supplementation for 1 year on bone metabolism in growing adolescents compared to controls. For the proposed study, a randomized double-blinded placebo controlled clinical trial will be conducted in 236 healthy adolescents aged 10-13 years, equally randomly assigned to one of four intervention groups: SCF (12 g/d), SCF + calcium (12 g/d of SCF + 600 mg/d of elemental calcium), placebo (0 g/d of SCF or of calcium), and placebo + calcium (0 g/d of SCF + 600 mg/d of elemental calcium); all administered twice a day. Bone mass will be assessed at baseline at 6 months and at 12 months and bone related biomarkers and fecal microbiome will be assessed at baseline and at 12 months.

Detailed Description

The adolescent period is crucial for optimizing future bone health because bone accumulates rapidly during these years and accounts for up to half of adult peak bone mass. Calcium intake during this period is critical for adequate bone mineralization but this is the nutrient most deficient in the diets of adolescents. A strategy to maximize bone mineralization during this critical period is to increase the absorption of the calcium being consumed. This could be achieved by supplementing diets with certain non-digestible carbohydrates, such as soluble corn fiber (SCF).

The primary aim of this study is to determine the effects of SCF supplementation for 1 year on bone metabolism in growing adolescents compared to controls. A randomized double-blinded placebo controlled clinical trial will be conducted in 236 healthy adolescents aged 10-13 years, equally randomly assigned to one of four intervention groups: SCF (12 g/d), SCF + calcium (12 g/d of SCF + 600 mg/d of elemental calcium), placebo (0 g/d of SCF or of calcium), and placebo + calcium (0 g/d of SCF + 600 mg/d of elemental calcium); all administered twice a day. Bone mass will be assessed at baseline at 6 months and at 12 months and bone related biomarkers and fecal microbiome will be assessed at baseline and at 12 months. The treatments will be provided in the form of flavored drinks twice per day (this was previously used in a short-term study among adolescents with excellent acceptance). Bone mass and anthropometric measurements will be assessed at baseline, at 6 months and at 12 months and bone related biomarkers and fecal microbiome will be assessed at baseline and at 12 months. Assessment visits will be conducted in the Clinical Research Center of the Stempel School of Public Health at Florida International University. We will also have short home visits at 3 and 9 months to collect unused supplements and provide more supplements. In all visits, participants will complete several questionnaires, such as general health, diet and activity questionnaire. Compliance will be evaluated at all visits and also in real time using a study mobile app.

The primary outcome is change in bone mineral content (BMC) measured using Dual-energy X-ray absorptiometry (DXA)

The secondary outcomes include biochemical markers reflecting vitamin D status, calcium homeostasis and bone, such as serum calcium, phosphorus, creatinine, osteocalcin, carboxy-terminal collagen crosslinks (CTX), parathyroid hormone (PTH), bone specific alkaline phosphatase, 25-(OH) vitamin D3, 1,25-(OH)2 vitamin D3, and insulin growth factor 1 (IGF-I) and urinary calcium, phosphorus, creatinine and N-telopeptide cross-links.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
213
Inclusion Criteria
  • Body mass index between the 5th and 85th percentiles for age and sex based on the Centers for Disease Control Growth Charts.
Exclusion Criteria
  • Subjects with any chronic illness requiring regular medication use.
  • Those taking calcium supplements (>500 mg/d) and vitamin D supplements (>400 IU/d) on a regular basis

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Soluble Corn Fiber (SCF) + CalciumSoluble Corn Fiber (SCF) + CalciumThis group will receive 12 g/d of soluble corn fiber (SCF) + 600 mg/d of elemental calcium carbonate, administered twice a day
Placebo + calciumPlacebo + calciumThis group will receive a similar supplement without SCF + 600 mg/d of elemental calcium carbonate, administered twice a day
Soluble Corn Fiber (SCF) without calciumSoluble Corn Fiber (SCF) without CalciumThis group will receive 12 g/d of soluble corn fiber (SCF) + 600 mg/d of elemental calcium carbonate, administered twice a day
PlaceboPlaceboThis group will receive a similar supplement without SCF or calcium, administered twice a day
Primary Outcome Measures
NameTimeMethod
Change in bone mineral content (BMC)12 months

Participants will undergo a bone scan at baseline, at 6 months and at 12-months follow-up visits using a dual energy x-ray absorptiometry (DXA)

Change in total bone mineral density (BMD)-z score12 months

Participants will undergo a bone scan at baseline, at 6 months and at 12-months follow-up visits using a dual energy x-ray absorptiometry (DXA)

Secondary Outcome Measures
NameTimeMethod
Change in osteocalcin12 months

It will be measured at baseline and 12-months follow-up visits from a serum sample using standardized kits

Change in serum carboxy-terminal collagen crosslinks (CTX)12 months

I will be measured at baseline and 12-months follow-up visits using standardized kits

Change in bone alkaline phosphatase12 months

It will be measured at baseline and 12-months follow-up visits from a serum sample using standardized kits

Change in serum insulin growth factor 1 (IGF-I).12 months

It will be measured at baseline and 12-months follow-up visits from a serum sample using standardized kits

Change in vitamin D status12 months

Vitamin D status will be measured as serum 25(OH)D at baseline and 12-months follow-up visits using standard ELISA kits.

Change in spine BMC12 months

Participants will undergo a bone scan at baseline, at 6 months and at 12-months follow-up visits using a dual energy x-ray absorptiometry (DXA)

Change in serum calcium12 months

Serum calcium will be measured at baseline and 12-months follow-up visits using standardized kits

Change in serum creatinine12 months

It will be measured at baseline and 12-months follow-up visits from a serum sample using standardized kits

Change in total BMD12 months

Participants will undergo a bone scan at baseline, at 6 months and at 12-months follow-up visits using a dual energy x-ray absorptiometry (DXA)

Change in serum phosphate12 months

It will be measured at baseline and 12-months follow-up visits from a serum sample using standardized kits

Change in parathyroid hormone (PTH)12 months

It will be measured at baseline and 12-months follow-up visits from a serum sample using standardized kits

Change in urinary calcium12 months

It will be measured at baseline and 12-months follow-up visits from a 24-h urine sample using standardized kits

Change in urinary creatinine12 months

It will be measured at baseline and 12-months follow-up visits from a 24-h urine sample using standardized kits

Change in urinary N-telopeptide cross-links12 months

It will be measured at baseline and 12-months follow-up visits from a 24-h urine sample using standardized kits

Change in body fat12 months

Participants will undergo a bone scan at baseline, at 6 months and at 12-months follow-up visits using a dual energy x-ray absorptiometry (DXA)

Change in phylogenetic diversity of bacterial communities12 months

Participants will provide a fecal sample at baseline and at 12-months follow-up visits using 16S ribosomal RNA (or 16S rRNA) gene sequences

Change in bone specific alkaline phosphatase12 months

It will be measured at baseline and 12-months follow-up visits from a serum sample using standardized kits

Change in urinary phosphorus12 months

It will be measured at baseline and 12-months follow-up visits from a 24-h urine sample using standardized kits

Trial Locations

Locations (1)

Flroida International University

🇺🇸

Miami, Florida, United States

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