LED Photobiomodulation Improves Radial Artery Vascular Function in Vitro

Not Applicable

Completed

• Conditions: Vascular Dysfunction

• Registration Number: NCT07041697
• Lead Sponsor: University of Sao Paulo

Brief Summary

LED photobiomodulation therapy (PBMT) is widely recognized for its beneficial effects in several clinical conditions; however, its potential in modulating vascular function is not yet fully established. Objective: This study aimed to evaluate the application of PBMT by LED (light-emittion diode) using a combined red and near-infrared light spectrum, with a focus on improving the vascular function of the radial artery in vitro. Methodology: A total of 30 radial artery segments were studied and divided into three groups of 10 segments: the PBMT LED group combined with red and infrared light (R + IR), the red light (R) group, and the infrared light (IR) group. Prior to light irradiation at wavelengths of 650 nm and 940 nm, vascular function in these segments was pharmacologically inhibited using L-NAME. The drugs phenylephrine, acetylcholine, and sodium nitroprusside were used to determine if irradiation could restore the inhibited vascular functions. Superoxide anion levels, an important reactive oxygen specie (ROS), were measured using a chemiluminescence assay with lucigenin. The bioavailability of nitric oxide was evaluated using a Griess Reagent Kit assay, while nitric oxide production was assessed through the DAF fluorescence assay. The vascular inflammation was determined by the Intercellular Adhesion Molecule 1 (ICAM-1) expression.

Detailed Description

Not available

Study Timeline

• Study Start: 2023-01-12
• Primary Completion: 2024-12-21
• Study Completion: 2025-02-21
• Study First Submitted: 2025-04-25
• Status Verified: 2025-06
• Study First Submitted QC: 2025-06-18
• Last Update Submitted: 2025-06-18
• Study First Posted: 2025-06-27
• Last Update Posted: 2025-06-27

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

• Patients undergoing coronary artery bypass grafting using the radial artery

Exclusion Criteria

• Patients with poorly compliant radial arteries or those who underwent coronary artery bypass grafting without use of the radial artery were excluded from the study

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Diameter of Isolated Radial Artery Rings (2 mm)	12 months	Description of Primary Outcome Measure(s): The primary outcome of this study is the evaluation of vascular compliance during vasodilation of isolated radial artery rings (2 mm in diameter), with a specific focus on the effects of LED photobiomodulation on arterial function.

Secondary Outcome Measures

Name	Time	Method
Evaluation of reactive oxygen species (ROS)	12 months	Superoxide anion (O₂--) generation, a key reactive oxygen species (ROS), will be measured in arterial tissue from the R + IR, R, and IR groups using a lucigenin-enhanced chemiluminescence assay. Lucigenin will serve as the electron acceptor, and nicotinamide adenine dinucleotide phosphate (NADPH) as the substrate. Results will be expressed as a percentage of concentration.

Trial Locations

Locations (1)

University of São Paulo Medical School in Ribeirão Preto; 🇧🇷 Ribeirão Preto, SP, Brazil