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Evaluation, in Humans, of the Correlation Between Hepatotoxicity, Neurotoxicity Induced by Oxaliplatin, and Blood Levels of HMGB1

Not Applicable
Recruiting
Conditions
Pancreatic Cancer
Resectable Pancreatic Adenocarcinoma
Adenocarcinoma
Resectable Esophageal Cancer
Resectable Gastric or Gastroesophageal Junction Adenocarcinoma
Oesophagogastric Cancer
Interventions
Biological: assess the serum HMGB1 concentrations before and after an oxaliplatin-based chemotherapy
Registration Number
NCT06649474
Lead Sponsor
University Hospital, Clermont-Ferrand
Brief Summary

Oesogastric and pancreatic adenocarcinomas are poor-prognosis cancers. Incidence of pancreatic cancer drastically increases to such an extent that it will become the second cause of cancer's mortality by 2030. A major challenge is to optimize the therapies for localized setting, when oxaliplatin-based chemotherapy is the standard, before and after surgical excision. Because in 50% of cases oxaliplatin triggers a grade 2-3 sinusoidal obstruction syndrome (SOS) which increases post-operative morbidity, decreases histological response to chemotherapy, increases tumor recurrence, and aggravates the risk of chemotherapy-induced peripheral neuropathy (CIPN).

There is an urgent need to better understand the biological processes involved in SOS, in order to prevent and treat it without stopping or reducing oxaliplatin administration.

The biological link between oxaliplatin and SOS has not been described, but recent murine experiments argue for HMGB1 to be the mediator released after exposure to oxaliplatin and inducing SOS, and thereafter CIPN. To date, no biomarker is established between murine and patient analyses, and the release of HMGB1 after oxaliplatin treatment and its effect on hepatic parenchyma is not described in patients. Investigators hypothesized is that HMGB1 would also been increased in patients after oxaliplatin treatment, and correlated to the development of SOS and CIPN. If confirmed, personalized treatment will be possible to target this pathway.

Therefore, investigators propose to dynamically explore this hypothesis in localized oesogastric and pancreatic cancer patients who will be routinely managed by an initial laparoscopy and post-oxaliplatin surgical excision.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
100
Inclusion Criteria
  • ECOG WHO Performance status = 0 or 1
  • Signed and dated informed consent
  • Patients with histological diagnosis of oesogastric or pancreatic adenocarcinoma
  • Resectable tumors
  • Patients able to have a laparoscopy
  • In case of absence of peritoneal invasion on the laparoscopy, patient candidate to a chemotherapy schedule by FLOT or FOLFOX in perioperative setting for oesogastric adenocarcinoma, or FOLFIRINOX in perioperative setting for pancreatic adenocarcinoma
  • Registration in a national health care system (CMU included)
  • Patient speak and understand the french
Exclusion Criteria
  • Histology other than adenocarcinoma
  • Metastatic disease
  • History of previous treatment with oxaliplatine
  • History of systemic chemotherapy administration within 5 years prior to inclusion,
  • Patient with an non balanced progressive condition/disease (liver failure, renal failure (creatinine clearance <30mL/min), respiratory failure, congestive heart failure, myocardial infarction in the last 6 months, etc.),
  • Patient on curative dose anticoagulant,
  • Patient with complete dihydropyrimidine dehydrogenase deficiency (Uracilemia ≥ 150 ng/ml),
  • Patient not operable for the pathology concerned,
  • Pregnant or breastfeeding woman, woman of childbearing age who has not performed a pregnancy test before the procedure,
  • Patient with legal incapacity (person deprived of liberty or under curatorship, stutorship, safeguard of justice),
  • Patient who, for psychiatric, social, family or geographical reasons, cannot be followed and/or comply with the requirements of the study,,
  • Discovery of peritoneal invasion during the peritoneal exploratory of the laparoscopy

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
comparate the Serum HMGB1 concentrations between patients with grade <2 SOS and those with grade 2,3assess the serum HMGB1 concentrations before and after an oxaliplatin-based chemotherapy-
Primary Outcome Measures
NameTimeMethod
Serum HMGB1 concentrationsAt exploratory laparoscopy and at the surgery

Assess Serum HMGB1 concentrations in two groups of patients : with grade 0 or 1 SOS and with grade ≥2 SOS, at two timepoints: before exploratory laparoscopy and before surgical excision to determinate the effect of HMGB1 on SOS development

Secondary Outcome Measures
NameTimeMethod
variation of serum HMGBI concentration (ng/ml)at the exploratory laparoscopy, at the first (Day1) and last cycle (assessed up to 75 days) of chemotherapy before surgery (each cycle=14 days) and at the surgery

mesurated the serum HMGB1 concentration (ng/ml) at several time of treatment of the patient then comparated these assess the dynamic variation of serum HMGB1 concentrations after oxaliplatin-based chemotherapy in oesogastric and pancreatic cancer patients with grade 0 or 1 SOS and with grade ≥2 SOS

variation of serum RAGE (receptor of HMGBI) concentrationat the exploratory laparoscopy, at the first (Day1) and last cycle (assessed up to 75 days) of chemotherapy before surgery (each cycle=14 days) and at the surgery

mesurated the serum RAGE concentration (ng/ml) at several time of treatment of the patient then comparated these assess the dynamic variation of serum HMGB1 concentrations after oxaliplatin-based chemotherapy in oesogastric and pancreatic cancer patients with grade 0 or 1 SOS and with grade ≥2 SOS

sinusoidal obstruction syndrome (SOS) diagnosisbefore to began chemotherapy and from 15 to 21 days after the last cycle of chemotherapy before surgery

compare SOS histology diagnosis (absent, mild, moderate, severe)) with splenic's volumetric measured by radiological's assessment

Evaluated the chemotherapy-induced peripheral neuropathy (CIPN)at the first (Day1) and last cycle (assessed up to 75 days) of chemotherapy before surgery (each cycle=14 days); at the surgery and at the first cycle (D1) then last cycle (assessed up to 75 days) after surgery

the CIPN will be evaluate by each patient who completed the visual analog score for pain

Predictive value of HMGB1 for the development of SOS and CIPNfrom exploratory laparoscopy to last cycle of chemotherapy after surgery assessed up to 10 month

Correlate CIPN, neuropathic pain with SOS, HMGB1, and RAGE measurements

Trial Locations

Locations (1)

CHU Estaing de Clermont-Ferrand

🇫🇷

Clermont-Ferrand, France

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