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Autonomic Cardiovascular Control in Response to Blood Volume Reduction in Blood Donors

Completed
Conditions
Autonomic Dysfunction
Blood Loss
Autonomic Imbalance
Interventions
Device: Monitoring of the autonomic nervous system
Registration Number
NCT04499664
Lead Sponsor
Hvidovre University Hospital
Brief Summary

The function of the autonomic nervous system can be assessed using baroreflex sensitivity (BRS) and heart rate variability (HRV). Decreased HRV has been shown to be predictive of morbidity and mortality in diverse medical conditions such as acute myocardial infarction, aneurysmal subarachnoid haemorrhage, autoimmune diseases, sepsis and surgery.

The function of the autonomic nervous system has not yet been investigated in a "pure hypovolemia" model. The aim of the current study is therefore to investigate and describe the function of the autonomic nervous system prior to, during and after reduction of blood volume in healthy blood donors.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
25
Inclusion Criteria
  • Volonteers eligable for blood donation following the Danish legislation
  • Male
  • Age 30-45
  • Written informed consent
  • Speak and understand Danish
Exclusion Criteria

Volonteers not eligable for blood donation following the Danish legislation, among others due to:

  • Alcohol and drug abuse
  • Cognitive dysfunction
  • Use of anxiolytic or antipsychotic drugs
  • Arrhythmias or heart failure
  • Diabetes mellitus type I
  • Diabetes mellitus type II
  • Use of opioids
  • History of following diseases in the autonomic nervous system: Parkinson disease, multiple sclerosis, autonomic neuropathies
  • History of cerebral apoplexy or transitory cerebral ischemia
  • Dementia
  • American Society of Anesthesiologists (ASA) score ≥ 4

Furthermore:

  • History of orthostatic intolerance and/or orthostatic hypotension
  • Use of following vasodilator antihypertensive drugs: beta-blockers, angiotensin converting enzyme inhibitors (ACEI), angiotensin 2 receptor blockers (ARBs), calcium channel blockers
  • Use of loop diuretics, thiazid diuretics and potassium-sparing diuretics

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Blood donorsMonitoring of the autonomic nervous systemHealhy young male bloddonors, aged 30-45
Primary Outcome Measures
NameTimeMethod
Changes in root mean square of successive NN-interval differences (RMSSD) during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in ms

Secondary Outcome Measures
NameTimeMethod
Changes in low frequency (LF) power during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in ms\^2

Changes in low frequency (LF) power during sleepThe night before and night after blood donation

Measured in ms\^2

Changes high frequency (HF) power during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in ms\^2

Changes high frequency (HF) power during sleepThe night before and night after blood donation

Measured in ms\^2

Changes in ratio of LF-to-HF power during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in %

Changes in ratio of LF-to-HF power during sleepThe night before and night after blood donation

Measured in %

Changes in root mean square of successive NN-interval differences (RMSSD) during sleepThe night before and night after blood donation

Measured in ms

Changes in standard deviation of N-N intervals (SDNN) during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in ms

Changes in standard deviation of N-N intervals (SDNN) during sleepThe night before and night after blood donation

Measured in ms

Percentage of successive RR intervals that differ by more than 50 ms (pNN50) during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in %

Percentage of successive RR intervals that differ by more than 50 ms (pNN50) during sleepThe night before and night after blood donation

Measured in %

Changes in S (area of the ellipse which represents total HRV) during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in ms

Changes in S (area of the ellipse which represents total HRV) during sleepThe night before and night after blood donation

Measured in ms

Changes in SD1 (Poincaré plot standard deviation perpendicular the line of identity) during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in ms

Changes in SD1 (Poincaré plot standard deviation perpendicular the line of identity) during sleepThe night before and night after blood donation

Measured in ms

Changes in SD2 (Poincaré plot standard deviation along the line of identity) during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in ms

Changes in SD2 (Poincaré plot standard deviation along the line of identity) during sleepThe night before and night after blood donation

Measured in ms

Changes in SD1/SD2 ratio during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in ms

Changes in SD1/SD2 ratio during sleepThe night before and night after blood donation

Measured in ms

Changes in systolic arterial pressure (SAP) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in mmHg by non-invasive LiDCO

Changes in diastolic arterial pressure (DAP) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in mmHg by non-invasive LiDCO

Changes in mean arterial pressure (MAP) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in mmHg by non-invasive LiDCO

Changes in pulse pressure (PP) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in mmHg by non-invasive LiDCO

Changes in heart rate (HR) during mobilisation15 minutes prior to and 15 minutes after blood donation

Measured in beats min-1 by non-invasive LiDCO

Changes in cardiac output (CO) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in L/min by non-invasive LiDCO

Changes in stroke volume (SV) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in mL by non-invasive LiDCO

Changes in systemic vascular resistance (SVR) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in dynes s cm-5 by non-invasive LiDCO

Changes in cerebral perfusion (ScO2) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in % by Root Masimo

Changes in muscular perfusion (SmO2) during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in % by Root Masimo

Changes in peripheral index during mobilization15 minutes prior to and 15 minutes after blood donation

Measured in % by Root Masimo

Concentration of C-reacitve proteinAt time of blood donation

Measured in gr/dL

V-PLEX Biomarker 54.Plex KitBlood taken at time of blood donation

54 key analytes that are important in inflammation response and immune system regulation as well as numerous other biological processes

Trial Locations

Locations (1)

Hvidovre University Hospital

🇩🇰

Copenhagen, Denmark

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