Impact of a carbohydrate-reduced diet on the adaptive immunity state of healthy volunteers
Not Applicable
- Conditions
- Ketogenic diet, low-carb nutrition, immunonutrition, chronic inflammation, low grade inflammation
- Registration Number
- DRKS00023373
- Lead Sponsor
- Klinikum der Universität München Klinik für Anästhesiologie und Intensivmedizin
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete
- Sex
- All
- Target Recruitment
- 44
Inclusion Criteria
Written informed consent
Age = 18 years
Exclusion Criteria
Women during pregnancy and lactation
Current intake of glucocorticoids
Last intake of glucocorticoids > 7 days ago
Severe metabolic disorder (e.g. diabetes mellitus) or
Autoimmune diseases
Participation in a clinical trial within the last 30 days
Current participation in another research project or clinical trial
Personal relationship with the examiner (relatives)
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Alteration of mRNA expression of the proinflammatory cytokine IFNy in CD8+ T cells after a 3-week low-carb diet
- Secondary Outcome Measures
Name Time Method Alteration of further inflammatory parameters (pro- and anti-inflammatory), mRNA expression profiles in PBMC and whole blood: IL-2, IL-4, IL-6, IL-10, IL-17, IL-1beta, FOXP3, RORc, GATA3, T-bet, CTLA4), Protein: TruCulture whole blood stimulation<br><br>Change of inflammatory relevant mRNA and microRNA expression profiles by array analysis<br><br>Flow cytometric analyses of T cell activation and subpopulations<br><br>mitochondrial analyses: quantification, mitochondrial membrane potential, ROS production, protein quantification of respiratory chain complexes using Western blot, oxygen consumption and exratacellular acidification<br><br>Ketone body concentrations in blood via POCT measurement<br><br>Weight History<br><br>Microbiological analysis from stool samples<br><br>Analysis of the amino acid metabolic pathways (especially tryptophan, phenylalanine) in urine<br><br>Analysis of bile acid metabolites in serum