Mechanisms of remote ischemic preconditioning in humans

Not Applicable

Completed

• Conditions: Myocardial infarction, endothelial disease; Circulatory System

• Registration Number: ISRCTN59201440
• Lead Sponsor: Academic Medical Center (Netherlands)

Brief Summary

2015 results in: https://www.ncbi.nlm.nih.gov/pubmed/25716080 [added 17/01/2019]

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Completion: 2012-08-28
• Study Start: 2014-05-22
• Last Update Posted: 11 February 2019

Recruitment & Eligibility

• Status: Completed
• Sex: Male
• Target Recruitment: 10

Inclusion Criteria

Healthy male volunteers aged 18-45 years

Exclusion Criteria

1. Any cardiovascular, kidney, pulmonary or endocrine diseases
2. Alcohol or drug abuse
3. No informed consent

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
actate dehydrogenase enzyme (LDH) release in human umbilical vein endothelial cells measured at different time points in our in vitro model after incubation with the serum.

Secondary Outcome Measures

Name	Time	Method
<br> 1. We measured mitogen activated protein kinase ERK 1/2, protein kinase B (AKT) and hypoxia inducible factor (HIF) 1 alpha in the endothelial cells by the use of western blotting at time point T0 before remote preconditioning and T1 (after 45 minutes, directly after the remote conditioning protocol).<br> 2. We measured human vascular endothelial growth factor in the plasma of the volunteers also at T0 and T1.<br>