(KIWI) Speeding up a Slow Protein for Muscle Mass With Hay Kiwifruit
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 13
- 试验地点
- 2
- 主要终点
- Whole Body Net Balance Measured by Gas Chromatography-Mass Spectrometry (GC-MS)
研究概览
简要总结
The specific hypothesis is that the consumption of 2 Hayward green kiwifruit (containing actinidin protease) prior to 100g of ground beef will increase the rate of protein digestion from the beef in the elderly, leading to an increased uptake of the essential amino acids. Furthermore, this increased essential amino acid availability will produce a greater postprandial net anabolic protein response, as well as increased fractional synthetic rates of muscle proteins.
详细描述
The specific hypothesis is that the consumption of 2 Hayward green kiwifruit (containing actinidin protease) prior to 100g of ground beef will increase the rate of protein digestion from the beef in the elderly, leading to an increased uptake of the essential amino acids. Furthermore, this increased essential amino acid availability will produce a greater postprandial net anabolic protein response, as well as increased fractional synthetic rates of muscle proteins.
To test this hypothesis, the investigators will use a double-blinded crossover design involving two intervention arms; in both arms 100g of ground beef (cooked weight) will be consumed following either 2 Hayward green kiwifruit (containing actinidin protease) or 2 Hort16A Gold kiwifruit (devoid of actinidin protease) by elderly subjects.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Other
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
盲法说明
Biological samples were coded and code list password protected until all data was analyzed.
入排标准
- 年龄范围
- 60 Years 至 85 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •men and women ages 60-85 inclusive.
排除标准
- •Inability to chew meats or difficulty swallowing solid foods
- •History of diabetes
- •History of malignancy in the 6 months prior to enrolment
- •History of gastrointestinal reduction or bypass surgery (Lapband, etc)
- •History of a chronic inflammatory condition or disease (Lupus, HIV'AIDS, etc)
- •History of chronic kidney disease or currently requiring dialysis.
- •Allergy to beef or kiwifruit
- •Subjects who do not or will not eat animal proteins
- •Subjects who cannot refrain from consuming protein or amino acid supplements during their participation in this study
- •Subjects who report regular resistance exercise (more than once per week)
- •Hemoglobin less than 9.5mg/dL at the screening visit
- •Platelets less than <150,000 at the screening visit
- •Subjects who are not willing or able to suspend aspirin for several days prior to their muscle biopsies.
- •Subjects who have been prescribed a blood-thinning medication (Coumadin, lovenox, heparin, Plavix, etc).
- •Concomitant use of corticosteroids (ingestion, injection or transdermal)
- •Any other disease or condition that would place the subject at increased risk of harm if they were to participate, at the discretion of the study physician
结局指标
主要结局
Whole Body Net Balance Measured by Gas Chromatography-Mass Spectrometry (GC-MS)
时间窗: 300 minutes
Blood samples were analyzed to quantify the appearance of the stable isotopes: 2H2-tyrosine, 2H4-tyrosine, and 2H5-phenylalanine in tracer to tracee ratio or mole percent excess. Changes in these markers over time enable the calculation of whole body protein metabolism. Calculations are done as follows: Total plasma rate of appearance = infusion rate/enrichment Fractional rate of appearance of tyrosine from phenylalanine= 2H4-tyrosine/2H5-phenylalanine Phenylalanine hydroxylation = fractional rate of appearance of tyrosine from phenylalanine x rate of appearance of tyrosine Protein Synthesis = \[(rate of appearance of phenylalanine -Phe hydroxylation) x 25\] Protein Breakdown = \[(rate of appearance of phenylalanine - phenylalanine infusion rate) x 25 - phenylalanine intake\] Net Balance = Protein Synthesis - Protein Breakdown
次要结局
- Change in Skeletal Muscle Protein Metabolism Measured by Gas Chromatography-Mass Spectrometry (GC-MS)(9.5 hours)