To Exam the Effects of Phyllanthus Niruri Extracts on Human Neutrophils

Completed

• Conditions: Neutrophil; Phyllanthus Abnormis Poisoning

• Registration Number: NCT05206058
• Lead Sponsor: Chang Gung Memorial Hospital

Brief Summary

To exam the effects of Phyllanthus niruri extracts(corilagin, phyllanthin and brevifolin) on human neutrophils

Detailed Description

Phyllanthus niruri extracts have anti-inflammatory effects in various cellular systems (monocytes, neutrophil). However, the underlying effects and mechanisms of Phyllanthus niruri extracts on neutrophils have not yet been investigated in detail.

In this study, first we would like to systemic exam the effects of corilagin, phyllanthin and brevifolin, three major active components, on human neutrophils.

Further to clarify whether Phyllanthus niruri extracts have any effect on neutrophils that may provide some insights on the ability of these compounds to modulate the innate immune response in acute organ injury.

Study Timeline

• Status Verified: 2022-01
• Study Start: 2022-01-01
• Study First Submitted: 2022-01-23
• Study First Submitted QC: 2022-01-23
• Study First Posted: 2022-01-25
• Primary Completion: 2022-09-30
• Study Completion: 2023-07-31
• Last Update Submitted: 2023-08-31
• Last Update Posted: 2023-09-01

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 240

Inclusion Criteria

• Healthy volunteers who aged 20-35 years.
• Do not have coagulopathy, systemic infection disease and severe liver and renal function impairment
• Accept venipuncture

Exclusion Criteria

• Healthy volunteers who aged over 35 years.
• Have coagulopathy, systemic infection disease and severe liver and renal function impairment

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Measurement of superoxide anion release	After neutrophil isolation, an average of 3 months	Superoxide anion released from human neutrophils were determined by measuring ferricytochrome c reduction.The neutrophils were activated with fMLF (30 nM) and cytochalasin B(0.5 μg/mL) (fMLF/CB). Changes in absorbance that occurred at 550 nm were observed continuously using a double beam spectrophotometer. Superoxide anion level was calculated using the methods described in previous report.
Measurement of intracellular ROS formation	After neutrophil isolation, an average of 3 months	Neutrophil ROS production was determined from the conversion of non-fluorescent DHR 123 to fluorescent rhodamine 123, detected using flow cytometry. Neutrophils (2 × 106 cells/ml) were incubated with DHR 123 (2 μM) for 15 min at 37°C, and then treated with honokiol (0.1-10 μM) for 5 min before the addition of fMLP/CB (0.5 μg/ml) for a further 5 min. The change in fluorescence was analysed using flow cytometry

Trial Locations

Locations (1)

Chang Gung Memorial Hospital; 🇨🇳 Taoyuan, Taiwan