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Inhaled Recombinant Non-immunogenic Staphylokinase vs Placebo in Patients With COVID-19 - FORRIF Trial

Phase 2
Terminated
Conditions
COVID-19
Interventions
Registration Number
NCT05135546
Lead Sponsor
Supergene, LLC
Brief Summary

Objective: to evaluate the tolerability, safety and efficacy of inhaled usage of the Recombinant Non-immunogenic Staphylokinase (Fortelyzin®) vs placebo in patients with COVID-19.

Detailed Description

Fortelyzin® (the active substance Forteplase) is a recombinant non-immunogenic staphylokinase with high fibrinselective thrombolytic activity. In a multicentre, randomised clinical trial in patients with ST-segment elevation myocardial infarction (FRIDOM), non-immunogenic staphylokinase was administered as a single intravenous bolus of 15 mg in all patients, regardless of bodyweight, and showed similar high reperfusion patency and fewer minor bleeding events compared with tenecteplase, as well as the absence of neutralising IgGs. Results of the multicentre, randomised clinical trial in patients with an acute ischaemic stroke (FRIDA) suggested that the non-immunogenic staphylokinase administrated as a single intravenous bolus of 10 mg in all patients within the 4-5 h after the onset of symptoms is non-inferior to alteplase. Mortality, symptomatic intracranial haemorrhage, and serious adverse events did not differ between treatment groups (Gusev EI, Martynov MYu, Nikonov AA et al. Non-immunogenic recombinant staphylokinase versus alteplase for patients with acute ischaemic stroke 4-5 h after symptom onset in Russia (FRIDA): a randomised, open label, multicentre, parallel-group, non-inferiority trial. Lancet Neurol. 2021; 20(9): 721-728).

Complex coagulation and hematologic abnormalities, including significantly elevated D-dimer and fibrin/fibrinogen values are the distinct features identified in severe SARS-CoV-2. In the list of antithrombotic therapy drugs in conjunction with anticoagulant and antiplatelet therapy in patients with COVID-19, published by Liverpool Drug Interactions Group, fibrinolytic therapy is also included.

So the main objectives of this study are to assess the tolerability, safety and efficacy of inhaled usage of fibrinolytic agent the recombinant non-immunogenic staphylokinase (Fortelyzin®) in patients with COVID-19.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
4
Inclusion Criteria
  • Men and women aged 18 and over

  • Clinical status according to the WHO scale - 6, 7, 8 and 9 points.

  • Verified respiratory infection COVID-19 by real-time PCR (quantitative)

  • Patient consent to use reliable contraceptive methods throughout the study and for 3 weeks after:

    • women who have a negative pregnancy test and use the following contraceptives: intrauterine devices, oral contraceptives, contraceptive patch, prolonged injectable contraceptives, double barrier method of contraception. Women who are not fertile can also take part in the study (documented conditions: hysterectomy, tubal ligation, infertility, menopause for more than 1 year);
    • men using barrier contraception. The study may also involve men who are not fertile (documented conditions: vasectomy, infertility)
  • Availability of signed and dated informed consent of the patient to participate in the study.

Exclusion Criteria
  • Clinical status according to the WHO scale - 1, 2, 3, 4 and 5 points.

  • Increased risk of bleeding:

    • extensive bleeding at the present time;
    • intracranial (including subarachnoid) hemorrhage at the present time.
  • Lactation, pregnancy

  • Known hypersensitivity to Fortelyzin®.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
ExperimentalRecombinant nonimmunogenic staphylokinaseRecombinant nonimmunogenic staphylokinase lyophilisate for preparation of a solution for inhaled administration, 5 mg (745,000 IU) complete with a solvent. 15 mg (2,235,000 IU) - 3 vials, regardless of body weight.
Placebo controlPlaceboPlacebo
Primary Outcome Measures
NameTimeMethod
Number of patients discharged from the hospital with clinical status according to the WHO Ordinal Scale For Clinical Improvement - 3 points or less28 days post randomization

The efficacy is evaluated in terms of the number of patients discharged from the hospital with clinical status according to the WHO Ordinal Scale For Clinical Improvement - 3 points or less

Secondary Outcome Measures
NameTimeMethod
Hospital length of stay28 days post randomization

The efficacy is evaluated in terms of the hospital length of stay

Number of oxygen support-free days28 days post randomization

The efficacy is evaluated in terms of the number of oxygen support-free days

Number of ventilation-free days28 days post randomization

The efficacy is evaluated in terms of the number of ventilation-free days

SpO2 level7, 14 and 28 days post randomization

The efficacy is evaluated in terms of the SpO2 level

Number of patients discharged from the hospital with clinical status according to the WHO Ordinal Scale For Clinical Improvement - 3 points or less14 days post randomization

The efficacy is evaluated in terms of the number of patients discharged from the hospital with clinical status according to the WHO Ordinal Scale For Clinical Improvement - 3 points or less

Number of ICU-free days28 days post randomization

The efficacy is evaluated in terms of the number of ICU-free days

Trial Locations

Locations (2)

City Clinical Hospital No.52

🇷🇺

Moscow, Russian Federation

N.V. Sklifosovsky Research Institute of Emergency Medicine

🇷🇺

Moscow, Russian Federation

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