The effects of Human neutrophil peptides (HNPs) on dermal fibroblasts and keratinocytes in 2D and 3D cell cultures
Not Applicable
Completed
- Conditions
- Healthy children
- Registration Number
- TCTR20200910003
- Lead Sponsor
- Institutional Review Board, Faculty of Medicine, Chulalongkorn University
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Male
- Target Recruitment
- 20
Inclusion Criteria
Healthy children who are circumcised at King Chulalongkorn Memorial Hospital, Bangkok, Thailand
Exclusion Criteria
Children who are unhealthy such as immunodeficiency and infection
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method HNP1-3 on human dermal fibroblast and keratinocyte proliferation 24 hrs Methylene blue staining and LDH cytotoxicity assay ,HNP1-3 on collagen type I production in dermal fibroblast and claudin-1 expression in keratinocyte 48 hrs ELISA and western blot
- Secondary Outcome Measures
Name Time Method HNP1-3 on collagen type I production in dermal fibroblast and claudin-1 expression in keratinocyte 48 hrs ELISA and western blot