Influence of Increased Sedentary Time on Long-term Rates of Skeletal Muscle Protein Synthesis in Young Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 8
- 试验地点
- 2
- 主要终点
- Muscle protein synthesis (FSR %/day)
研究概览
简要总结
This study will investigate the influence of increased sedentary time on long-term measures of muscle protein synthesis and metabolic health. The investigators will test the hypothesis that increased time spent in sedentary behaviours will lead to a reduction in long-term measures of muscle protein synthesis and compromised metabolic health.
详细描述
Sarcopenia, the loss of muscle mass with age, is thought to be accelerated by an inactive, sedentary lifestyle. Increased sedentary time has consistently been associated with lower muscle mass and compromised metabolic health. However, there is currently a lack of direct evidence to support these associations.
Therefore, this study will investigate whether increased sedentary time (reduced step count and exercise cessation) directly influences long-term measures of muscle protein synthesis and metabolic health in young, active adults. Following a 7 day period of normal habitual physical activity, participants will undertake a 7 day period of step reduction and exercise cessation. It is hypothesised that 7 days of increased sedentary time will result in a reduction in long-term rates of muscle protein synthesis and a worsening of metabolic health.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Body mass index (18.5-29.99 kg/m2)
- •Active - defined as: ≥ 7000 steps per day (assessed during screening) and taking part in regular exercise (defined as participation in ≥ 3 sessions of aerobic and/or resistance exercise type sessions a week for ≥ 6 months prior to recruitment)
- •Good general health
排除标准
- •Lidocaine allergy
- •Hypertension (≥140/90 mmHg)
- •Current participation in another clinical study
- •Previous participation in this study
- •Bleeding disorder/s
- •Current or recent smoker
- •Past history of substance abuse and/or taking prescription or non-prescription medication (e.g., beta-blockers, insulin or thyroxine) or supplements that may influence normal metabolic responses.
- •Participants who have previously (within 5 years of the present study) had 4 or more muscle biopsies obtained from the thigh quadriceps region will be ineligible.
研究组 & 干预措施
Physical inactivity
Study arm consisting of 7 days of habitual physical activity followed by 7 days of step reduction and exercise cessation.
干预措施: 7 days of habitual physical activity followed by 7 days of reduced physical activity (Behavioral)
结局指标
主要结局
Muscle protein synthesis (FSR %/day)
时间窗: 0-14 days
Long-term muscle protein synthesis (FSR %/day) will be determined using deuterium oxide (D2O) to compare muscle protein synthesis rates over the first 7 day period and the second 7 day period.
次要结局
- Rate of fat oxidation at rest (g/min)(For 20 minutes at day 7 and day 14)
- Rate of fat oxidation during an oral glucose tolerance test (g/min)(At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14)
- Blood glucose concentration(At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14)
- Blood insulin concentration(At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14)
- Rate of carbohydrate oxidation during an oral glucose tolerance test (g/min)(At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14)
- Blood fatty acid concentration(At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14)
- Rate of carbohydrate oxidation at rest (g/min)(For 20 minutes at day 7 and day 14)