Regulation of lipid metabolism by fructose versus glucose in obese humans with and without hepatic steatosis and its relation to insulin sensitivity

Completed

Conditions: metabolic syndrome
non-alcoholic fatty liver disease
10018424
10013317

Registration Number: NL-OMON40700

Lead Sponsor: Academisch Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 36

Inclusion Criteria

-eligible and scheduled for bariatric surgery (gastric bypass)
-18-65 years of age
-ability to provide informed consent
-stable weight 3 months prior to inclusion
-willingness to stop lipid lowering medication 4 weeks prior to start

Exclusion Criteria

-primary lipid disorder
-childhood onset obesity (i.e. <12 years of age)
-use of exogenous insulin, GLP1 agonists or DDP4 inhibitors
-all medica! and psychiatric conditions except for obesity-related diseases
-coagulation disorders
-uncontrolled hypertension (blood pressure >150/95 mmHg)
-renal insufficiency (plasma creatinin >150 umol/l)
-excessive alcohol intake (>14 units/week)
-pregnancy, breastfeeding
-contraindication to MR scanning (e.g. pacemaker, metallic foreign body, claustrophobia)

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Differences in regulation of lipogenic pathways in insulin-sensitive tissues by<br /><br>fructose versus glucose intake in obese humans with or without hepatic<br /><br>steatosis.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>-regulation of de novo lipogenesis (lipid flux) in response to oral fructose or<br /><br>glucose<br /><br>-the role of important lipogenic enzymes (such as ChREBP-/ and FGF21) in de<br /><br>novo lipogenesis, fat distribution and ectopic fat accumulation<br /><br>-the role of these pathways in peripheral and hepatic insulin resistance and<br /><br>endogenous glucose production-differences in lipid species composition in relation to gene expression<br /><br>profiles and lipid and glucose fluxes<br /><br>-differences in de novo lipogenesis, fat distribution and ectopic fat<br /><br>accumulation in relation to systemic and hepatic insulin resistance</p><br>