Optimizing the amount of dietary protein to maximally stimulate post-exercise muscle protein synthesis in elderly men.

Completed

Conditions: age-related muscle loss
Sarcopenia
10037008
10028302

Registration Number: NL-OMON41601

Lead Sponsor: niversiteit Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 75

Inclusion Criteria

Healthy males
Age between 55 and 80 y
BMI between 18.5 and 30 kg/m2

Exclusion Criteria

Celiac disease
Lactose intolerance
Smoking
Diabetes
Diagnosed GI tract diseases
Arthritic conditions
A history of neuromuscular problems
Any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
Participation in exercise program
Hypertension, high blood pressure that is above 140/90 mmHg.
Cancer
Cardiovascular diseases
Donated blood within the last 3 months

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main study endpoints are muscle protein synthesis (MPS) rates. In order to<br /><br>determine the MPS, the following parameters will be measured:<br /><br>• Muscle protein-bound L-[ring-2H5]-phenylalanine enrichment (expressed as MPE)<br /><br>• Muscle protein-bound L-[1-13C]-leucine enrichment (expressed as MPE)<br /><br>• Plasma L-[ring-2H5]-phenylalanine, L-[1-13C]-KIC,enrichments (expressed as<br /><br>MPE)</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secondary endpoints include whole-body protein metabolism (synthesis,<br /><br>breakdown, oxidation, and net balance) and measures of digestion and absorption<br /><br>kinetics. Therefore, the following parameters will be measured:<br /><br>• Plasma enrichments (in MPE) of:<br /><br>o L-[ring-2H5]-phenylalanine<br /><br>o L-[ring-2H4]-tyrosine<br /><br>o L-[3,5-2H2]-tyrosine<br /><br>• Plasma total phenylalanine and tyrosine concentrations (expressed as µmol/L)<br /><br>• Total plasma amino acids (AAmax [µmol/L])<br /><br>• Plasma glucose (glucosemax [mmol/L])<br /><br>• Plasma insulin (insulinmax [mU/L])</p><br>