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Start Taking Action For TB Diagnosis

Not yet recruiting
Conditions
Tuberculosis
Diagnosis
Interventions
Diagnostic Test: Molecular Diagnostics
Diagnostic Test: Qualitative C-Reactive Protein (CRP)
Diagnostic Test: Urine lateral flow tests
Diagnostic Test: Portable Chest X Ray Image Acquisition
Registration Number
NCT05845112
Lead Sponsor
Liverpool School of Tropical Medicine
Brief Summary

Globally, tuberculosis (TB) is one of the main causes of death and the leading cause from a single infectious agent. In 2020, an estimated 9.9 million people developed TB and 1.5 million died. Millions of people remain undiagnosed with TB, hindering efforts to end TB. TB tests have inadequate accuracy or performance characteristics for implementation across all populations and settings. None of the tests meet the WHO-Target-Product Profile for TB screening and most need specialized laboratory staff and infrastructure, making them unsuitable for primary health care (PHC).

The overall aims for this project are to:

1. Accelerate the introduction/adoption of TB diagnostic tools and test combinations at PHC, for the timely detection of TB and improved linkage to treatment

2. Develop conditions for sustainable and equitable access to TB diagnostics tools and test combinations within PHC,

3. Strengthen global alliances and national partnerships to enable scale-up.

The study is split into two major Phases. This application is focused on Phase 1. Specific Phase 1 objectives are to:

4. Evaluate the performance of selected TB diagnostic tools, and

5. Identify TB test combinations that increase the proportion of people diagnosed with bacteriologically confirmed TB.

Methods in Brief: Activities will be conducted in Bangladesh, Brazil, Kenya, Cameroon, Malawi, Nigeria, and Vietnam.

Each country will study selected priority populations at risk of TB, including adults attending PHC centers and district hospitals; people living with HIV (PLHIV); marginalized populations (internally displaced, refugees and pastoralists), and children. Activities within countries will use standardized protocols for evaluating diagnostic tests and combinations.

Detailed Description

Tuberculosis (TB) is second only to the coronavirus infectious disease 2019 (COVID-19) as a single-species cause of adult infectious death worldwide1. Nearly four of the 10.6 million people estimated to develop TB each year are not diagnosed or treated. Moreover, an estimated 1.6 million people died of TB in 2021, with the large gap between TB diagnosis and treatment being a major contributor to mortality1-4. The World Health Organisation (WHO) has identified 30 high TB burden countries (TB burden, HIV-associated TB burden, multi-drug resistant/rifampicin resistant-TB) that are predominately low- or low-to-middle income, and together accounted for 86-90% of the estimated global TB incidence in 20191. Within these countries, and globally, TB disproportionally affects the poor, and most of the 'missed' cases occur among populations with limited access to healthcare (men, people living with HIV, residents of rural and remote settings and informal urban settlements and displaced populations5 and in children6.

The WHO End TB Strategy calls for a 90% reduction in TB deaths by 2030 compared to 2015, an 80% reduction in estimated TB incidence, and elimination of household catastrophic costs due to TB. Unfortunately, the 2021 WHO Global TB Report shows that we are very far from achieving these targets, and they will likely not be met without a step-change in efforts to improve early diagnosis and treatment of TB. Moreover, the COVID-19 pandemic severely disrupted TB diagnosis and treatment programs, hindering efforts to eliminate TB as a public health issue, and the ramifications of the pandemic are likely to be experienced by fragile health systems for many years7. New approaches to facilitate access to same-day, same-setting diagnosis and treatment of TB are urgently required.

TB detection in adults is mainly dependent upon passive case finding (PCF), requiring individuals to visit health facilities, be asked by health workers whether they have symptoms consistent with TB, and subsequently tested for TB. Only some countries use active case finding (ACF) and intensified case funding (ICF) as a complementary measure 8,9. However, this approach has considerable limitations. Exit interviews at clinics and simulated patient studies demonstrate that clinicians rarely ask about TB symptoms10-12, and even when patients volunteer symptoms, sputum testing is infrequently requested, and patients are often unable to produce sputum10. Thus, PCF is insufficient to identify most people with TB and needs to be supplemented with other approaches, such as intensified case finding in health facilities and ACF, which allows detection of people with infectious TB (and potentially asymptomatic TB; an estimated 50% of prevalent community cases14), speeding up diagnosis and linkage to treatment, and potentially reducing transmission. The location where people with presumptive TB are tested and the timeliness of diagnosis are also important. Approaches that rely on sputum transport networks or referral of patients to centralized laboratories often result in diagnostic delays and poor linkage to treatment, while local testing has been associated with increased numbers detected and confirmed and improved linkage to treatment15. TB diagnosis is also particularly challenging for well-defined key and vulnerable populations, including: people living in communities with very high HIV prevalence16; those living in informal urban settlements with poor access to health facilities and marginalized communities, such as refugees and nomadic groups; and in children.

Overall, for this study, countries have been selected to provide a broad representation from World Bank country development groups, global regions, covering South-East Asia, Africa, and the Americas, with a combined population of 780 million people. We have included countries with a high TB burden in the general population (Bangladesh, Brazil, Kenya, Nigeria); countries with a high burden of HIV-associated TB (Brazil, Cameroon, Kenya, Malawi, Nigeria); and countries with a high burden of drug resistant TB (Bangladesh, Nigeria, Vietnam)1. Data across partner countries, have shown that TB-affected households experienced health-related costs reaching 50% of their annual household income in Sub Saharan African countries such as Nigeria, Cameroon, Malawi and Kenya17-19. Given these findings, WHO's End TB Strategy included the goal of zero TB-affected families incurring catastrophic costs. These are defined as 20% of annual household income lost due to an episode of TB. Countries that have conducted formal patient cost surveys employing WHO tools and definitions such as Vietnam have measured catastrophic cost incurrence of 63% and 98% among drug susceptible (DS) DS-TB and multidrug resistant (MDR) MDR-TB households, respectively20. Thus, as diagnostics can play a critical role in optimizing the patient pathway, better diagnostic tools and algorithms may also positively impact performance against the End TB Strategy's socioeconomic indicator. In this project we will aim to demonstrate that combinations of current and newer TB tests can facilitate TB diagnostic testing in locations where it is not currently available; that the optimized use of tests at the point of need increases the proportion of people correctly diagnosed, increases access to TB treatment, and potentially reduces TB mortality and transmission.

The project will focus on key and vulnerable populations including in settings where TB diagnostic testing is not normally performed, and people are diagnosed clinically due to limited access to laboratory facilities. In Phase 1 we will identify new combinations of tests to provide same-day, same-setting diagnosis, and assess the feasibility of scaling-up and subsequent linkage to TB care that will be formally assessed in Phase 2 of the research.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
15300
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Bangladesh: Primary healthcare clinicsPortable Chest X Ray Image AcquisitionCountry with a high TB burden in the general population with recruitment to the study occurring at the PHC.
Brazil: Primary healthcare clinics MaceioMolecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Maceio.
Brazil: Primary healthcare clinics MaceioQualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Maceio.
Brazil: Primary healthcare clinics MaceioUrine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Maceio.
Bangladesh: Informal settlements active case findingQualitative C-Reactive Protein (CRP)Country with a high TB burden in the general population with recruitment to the study through active case finding targeting informal settlements.
Bangladesh: Informal settlements active case findingUrine lateral flow testsCountry with a high TB burden in the general population with recruitment to the study through active case finding targeting informal settlements.
Viet Nam: Informal settlements active case findingQualitative C-Reactive Protein (CRP)Country with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting informal settlements.
Malawi: Primary healthcare clinicsUrine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Blantyre, Malawi.
Cameroon: Informal settlements/rural poor active case finding (ACF)Urine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring through ACF in rural areas and informal settlements.
Brazil: Primary healthcare clinics AracajuQualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Aracaju.
Cameroon: Primary healthcare clinicsMolecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the PHC.
Cameroon: District HospitalQualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring at the District Hospital.
Cameroon: Informal settlements/rural poor active case finding (ACF)Molecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring through ACF in rural areas and informal settlements.
Cameroon: Informal settlements/rural poor active case finding (ACF)Qualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring through ACF in rural areas and informal settlements.
Nigeria: Primary healthcare clinicsMolecular DiagnosticsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at the PHC.
Brazil: Primary healthcare clinics AracajuPortable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Aracaju.
Brazil: Primary healthcare clinics MaceioPortable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Maceio.
Viet Nam: Informal settlements active case findingUrine lateral flow testsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting informal settlements.
Viet Nam: Informal settlements active case findingPortable Chest X Ray Image AcquisitionCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting informal settlements.
Viet Nam: ChildrenQualitative C-Reactive Protein (CRP)Country with a high burden of drug resistant TB infections with recruitment to the study occurring only in children, an under represented cohort in TB studies.
Cameroon: Primary healthcare clinicsQualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring at the PHC.
Cameroon: District HospitalPortable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring at the District Hospital.
Cameroon: childrenPortable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring only in children, an under represented cohort in TB studies.
Nigeria: Primary healthcare clinicsPortable Chest X Ray Image AcquisitionCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at the PHC.
Nigeria: District HospitalsMolecular DiagnosticsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at the District Hospital.
Viet Nam: ChildrenUrine lateral flow testsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring only in children, an under represented cohort in TB studies.
Viet Nam: ChildrenPortable Chest X Ray Image AcquisitionCountry with a high burden of drug resistant TB infections with recruitment to the study occurring only in children, an under represented cohort in TB studies.
Malawi: Primary healthcare clinicsMolecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Blantyre, Malawi.
Malawi: Primary healthcare clinicsPortable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Blantyre, Malawi.
Cameroon: Primary healthcare clinicsPortable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring at the PHC.
Cameroon: Primary healthcare clinicsUrine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the PHC.
Cameroon: childrenQualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring only in children, an under represented cohort in TB studies.
Cameroon: District HospitalMolecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the District Hospital.
Cameroon: District HospitalUrine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the District Hospital.
Cameroon: childrenUrine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring only in children, an under represented cohort in TB studies.
Nigeria: District HospitalsQualitative C-Reactive Protein (CRP)Country with a high burden of drug resistant TB infections with recruitment to the study occurring at the District Hospital.
Nigeria: Internally displaced people (IDP)/refugeesUrine lateral flow testsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting Internally displaced people (IDP)/refugees.
Nigeria: Internally displaced people (IDP)/refugeesPortable Chest X Ray Image AcquisitionCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting Internally displaced people (IDP)/refugees.
Cameroon: Informal settlements/rural poor active case finding (ACF)Portable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring through ACF in rural areas and informal settlements.
Cameroon: childrenMolecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring only in children, an under represented cohort in TB studies.
Nigeria: Primary healthcare clinicsUrine lateral flow testsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at the PHC.
Nigeria: NomadsQualitative C-Reactive Protein (CRP)Country with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting Nomad populations.
Nigeria: NomadsPortable Chest X Ray Image AcquisitionCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting Nomad populations.
Nigeria: Primary healthcare clinicsQualitative C-Reactive Protein (CRP)Country with a high burden of drug resistant TB infections with recruitment to the study occurring at the PHC.
Nigeria: Internally displaced people (IDP)/refugeesMolecular DiagnosticsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting Internally displaced people (IDP)/refugees.
Nigeria: District HospitalsUrine lateral flow testsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at the District Hospital.
Nigeria: District HospitalsPortable Chest X Ray Image AcquisitionCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at the District Hospital.
Nigeria: NomadsUrine lateral flow testsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting Nomad populations.
Kenya: Primary healthcare clinicsPortable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring at the PHC.
Bangladesh: District HospitalsMolecular DiagnosticsCountry with a high TB burden in the general population with recruitment to the study occurring at the District Hospital.
Bangladesh: Informal settlements active case findingPortable Chest X Ray Image AcquisitionCountry with a high TB burden in the general population with recruitment to the study through active case finding targeting informal settlements.
Brazil: Primary healthcare clinics AracajuMolecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Aracaju.
Nigeria: NomadsMolecular DiagnosticsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting Nomad populations.
Kenya: District HospitalMolecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the District Hospital.
Kenya: District HospitalQualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring at the District Hospital.
Bangladesh: Primary healthcare clinicsMolecular DiagnosticsCountry with a high TB burden in the general population with recruitment to the study occurring at the PHC.
Bangladesh: District HospitalsUrine lateral flow testsCountry with a high TB burden in the general population with recruitment to the study occurring at the District Hospital.
Bangladesh: Informal settlements active case findingMolecular DiagnosticsCountry with a high TB burden in the general population with recruitment to the study through active case finding targeting informal settlements.
Nigeria: Internally displaced people (IDP)/refugeesQualitative C-Reactive Protein (CRP)Country with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting Internally displaced people (IDP)/refugees.
Kenya: Primary healthcare clinicsMolecular DiagnosticsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the PHC.
Kenya: Primary healthcare clinicsQualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring at the PHC.
Bangladesh: Primary healthcare clinicsQualitative C-Reactive Protein (CRP)Country with a high TB burden in the general population with recruitment to the study occurring at the PHC.
Bangladesh: District HospitalsPortable Chest X Ray Image AcquisitionCountry with a high TB burden in the general population with recruitment to the study occurring at the District Hospital.
Viet Nam: Primary healthcare clinicsMolecular DiagnosticsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at Primary healthcare clinics.
Viet Nam: Primary healthcare clinicsQualitative C-Reactive Protein (CRP)Country with a high burden of drug resistant TB infections with recruitment to the study occurring at Primary healthcare clinics.
Viet Nam: Primary healthcare clinicsUrine lateral flow testsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at Primary healthcare clinics.
Viet Nam: ChildrenMolecular DiagnosticsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring only in children, an under represented cohort in TB studies.
Kenya: Primary healthcare clinicsUrine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the PHC.
Kenya: District HospitalUrine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the District Hospital.
Kenya: District HospitalPortable Chest X Ray Image AcquisitionCountry with a high burden of HIV associated TB with recruitment to the study occurring at the District Hospital.
Bangladesh: Primary healthcare clinicsUrine lateral flow testsCountry with a high TB burden in the general population with recruitment to the study occurring at the PHC.
Bangladesh: District HospitalsQualitative C-Reactive Protein (CRP)Country with a high TB burden in the general population with recruitment to the study occurring at the District Hospital.
Brazil: Primary healthcare clinics AracajuUrine lateral flow testsCountry with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Aracaju.
Viet Nam: Primary healthcare clinicsPortable Chest X Ray Image AcquisitionCountry with a high burden of drug resistant TB infections with recruitment to the study occurring at Primary healthcare clinics.
Viet Nam: Informal settlements active case findingMolecular DiagnosticsCountry with a high burden of drug resistant TB infections with recruitment to the study occurring through ACF targeting informal settlements.
Malawi: Primary healthcare clinicsQualitative C-Reactive Protein (CRP)Country with a high burden of HIV associated TB with recruitment to the study occurring at the Primary healthcare clinics in Blantyre, Malawi.
Primary Outcome Measures
NameTimeMethod
Primary study endpoint12 months

Generation of robust estimates of the diagnostic accuracy and performance of TB diagnostic tests and test combinations in primary healthcare settings and key and vulnerable populations.

Secondary Outcome Measures
NameTimeMethod
Secondary study endpoint (1)2 years

Estimates of the predicted performance of TB diagnostic test combinations and how they perform in specific populations.

Secondary study endpoint (2)2 years

Estimates of the predicted performance of TB diagnostic test combinations and how they perform in specific populations.

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