Rapid Detection of Pseudomonas Aeruginosa in Bronchoalveolar Lavage Fluid Using Label-free Single-particle Imaging Technology and Assessment of Post-treatment Efficacy in Patients With Pseudomonas Aeruginosa Infection

Not yet recruiting

• Conditions: Rapid Detection; Pseudomonas Aeruginosa Infection

• Registration Number: NCT06986512
• Lead Sponsor: The First Affiliated Hospital with Nanjing Medical University

Brief Summary

The aim of this study is to observe specimens of bronchoalveolar lavage fluid from patients using a reflection enhanced dark-field scattering microscopy. By observing parameters such as the size, morphology, scattering intensity, and movement speed of pathogens, combined with the clinical characteristics of patients, a rapid diagnosis of Pseudomonas aeruginosa infection can be made. At the same time, the bronchoalveolar lavage fluid samples of patients infected with Pseudomonas aeruginosa before and after treatment were compared, and the quantity and vitality of Pseudomonas aeruginosa were observed to judge the efficacy of antibiotics.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2025-03-11
• Status Verified: 2025-05
• Study First Submitted QC: 2025-05-22
• Study First Posted: 2025-05-23
• Last Update Submitted: 2025-06-26
• Last Update Posted: 2025-06-27
• Study Start: 2025-07-01
• Primary Completion: 2025-11-30
• Study Completion: 2025-11-30

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Aged 18 years and above
• After admission, Pseudomonas aeruginosa was cultured in bronchoalveolar lavage fluid or lower respiratory tract aspirates

Exclusion Criteria

• Age under 18 years old or over 90 years old
• Pregnancy, lactation period -

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
scattering intensity of pathogen	From enrollment to the end of treatment at 2 weeks	Determine the shape of pathogens through scattering intensity
movement speed of pathogens	From enrollment to the end of treatment at 2 weeks	Measure the movement speed of pathogens, such as flagellates like Pseudomonas aeruginosa, which have a fast movement speed
The size of pathogens	From enrollment to the end of treatment at 2 weeks	Measure the size of pathogens

Trial Locations

Locations (1)

The First Affiliated Hospital of Nanjing Medical University; 🇨🇳 Nanjing, Jiangsu, China