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A Clinical Trial to Study the Efficacy and Safety of Different Doses of Bone Marrow Derived Mesenchymal Stem Cells in Patients With Critical Limb Ischemia Due to Buergers Disease

Phase 2
Completed
Conditions
Buerger's Disease
Critical Limb Ischemia
Interventions
Biological: Allogeneic Mesenchymal Stem Cells
Registration Number
NCT01484574
Lead Sponsor
Stempeutics Research Pvt Ltd
Brief Summary

This is an open label, non-randomized, dose ranging study to evaluate the safety and efficacy of different doses of Stempeucel in critical limb ischemia patients.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
90
Inclusion Criteria
  • Buerger's disease as diagnosed by Shionoya criteria
  • Males or females (willing to use accepted methods of contraception during the course of the study) in the age group of 18-65 yrs
  • Established CLI in the study limb, clinically and hemodynamically confirmed as per Rutherford- III-5
  • Patients in Rutherford- III-6 if gangrene extending maximally up to the head of metatarsal but limited to toes (Patients with wet gangrene must undergo wound debridement / amputation before screening)
  • Patients having infrapopliteal occlusive disease with rest pain and ischemic ulcer/necrosis, who are not eligible for or have failed traditional revascularization treatment as per the investigators judgment (No option patients)
  • Ankle Brachial Pressure Index (ABPI) ≤ 0.6 or ankle pressure ≤ 50 mm Hg or TcPO2 ≤ 40 mmHg in the foot of the study limb
  • Patients who are able to understand the requirements of the study, and willing to provide voluntary written informed consent and video consent, abide by the study requirements, and agree to return for required follow-up visits
Exclusion Criteria
  • Patients with CLI indicated for major amputation during screening
  • Atherosclerotic PAD
  • Ulcers with exposure of tendon and/bone in the shin region
  • Previous above transmetatarsal amputation in study limb
  • Any Lumbar sympathectomy procedure performed less than 90 days prior to the screening
  • Patients with gait disturbance for reasons other than CLI
  • Diagnosis of diabetes mellitus (type 1 or type 2)
  • Patients having left ventricular ejection fraction < 35%
  • Patients suffering from clinically relevant peripheral neuropathy
  • History of Stroke or myocardial infarction
  • Patients who are contraindicated for MRA
  • Patients with deep vein thrombosis in any limb
  • Patients who have clinically serious and/or unstable inter-current infection, medical illnesses or conditions that are uncontrolled or whose control, in the opinion of the Investigator, may be jeopardized by participation in this study or by the complications of this therapy
  • Documented terminal illness or cancer or any concomitant disease process with a life expectancy of <1 year
  • Patients already enrolled in another investigational drug trial or completed within 3 months or those who have participated in any stem cell clinical trial
  • Patient with known hypersensitivity to the constituents of the IMP - dimethyl sulfoxide (DMSO) or human serum albumin (HSA)
  • History of severe alcohol or drug abuse within 3 months of screening
  • Hb% < 10 gm% for males, Hb% < 9 gm% for females, serum creatinine ≥ 2mg%, serum Total Bilirubin ≥2mg%
  • Pregnant and lactating women
  • Patients tested positive for HIV 1, HCV, HBV, CMV, RPR

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Low doseAllogeneic Mesenchymal Stem CellsStempeucel - CLI will be administered at the lowest dose
Intermediate doseAllogeneic Mesenchymal Stem CellsStempeucel - CLI will be administered at intermediate dose
Primary Outcome Measures
NameTimeMethod
Relief of the rest pain24 months

Rest pain will be measured using rest pain scale (0 to 10)

Healing of ulcerations or reduction of ulcer area in the target limb24 months

Complete ulcer healing defined as complete epithelisation of ulcer and partial ulcer healing as at least 30% decrease in ulcer size.

Secondary Outcome Measures
NameTimeMethod
Major amputation free survival6 and 24 months
Pain free walking distance6 and 24 months
Ankle brachial pressure index (ABPI) - measured by Doppler6 and 24 months
Increase in transcutaneous partial oxygen pressure (TcPO2)6 and 24 months
Quality of life by King's College VascuQOL questionnaire6 and 24 months
Angiogenesis - collateral blood vessels by Magnetic resonance angiogram (MRA)6 and 24 months
The type of adverse events AE(s), number of AE(s) and proportion of patients with AE(s).6 and 24 months
Assessment of clinical laboratory parameters6 and 24 months
Physical examination findings and assessment of vital signs6 and 24 months
Assessment of electrocardiogram (ECG) parameters6 and 24 months

Trial Locations

Locations (10)

Department of Vascular and Endovascular Surgery, M. S. Ramaiah Medical College and Hospitals

🇮🇳

Bangalore, Karnataka, India

Department of Surgical Disciplines, All India Institute of Medical Sciences

🇮🇳

New Delhi, Delhi, India

Division of Peripheral Vascular and Endovascular Sciences, Medanta - The Medicity

🇮🇳

Gurgaon, Haryana, India

Peripheral Vascular Surgery, Sri Jayadeva Institute of Cardiovascular Sciences & Research

🇮🇳

Bangalore, Karnataka, India

Department of Surgery, KMC, Mangalore

🇮🇳

Mangalore, Karnataka, India

Department of Vascular Surgery, AMRI Hospital

🇮🇳

Kolkata, West Bengal, India

Nightingale Hospital

🇮🇳

Kolkata, West Bengal, India

Department of Vascular Surgery, Madras Medical College

🇮🇳

Chennai, Tamil Nadu, India

Department of Vascular Surgery, Sri Ramchandra Medical College

🇮🇳

Chennai, Tamil Nadu, India

Department of Vascular Surgery, Stanley Medical College

🇮🇳

Chennai, Tamil Nadu, India

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