BFR and Muscle Mitochondrial Oxidative Capacity

Not Applicable

Completed

• Conditions: Strength; Hypertrophy; Mitochondria
• Interventions: Behavioral: Low Load Resistance Exercise + BFR; Behavioral: Low Load Resistance Exercise

• Registration Number: NCT03723226
• Lead Sponsor: Louisiana State University and A&M College

Brief Summary

Blood flow restricted (BFR) exercise has been shown to improve skeletal muscle adaptations to resistance exercise. BFR uses blood pressure cuffs (i.e., tourniquets) to reduce skeletal muscle blood flow during resistance exercise. One benefit of BFR is that skeletal muscle adaptations to resistance exercise training including muscle hypertrophy and increases in strength can be achieved at lower-loads (e.g., 25-30% 1RM), that are often comparable to more traditional resistance training loads (70-85% 1RM). However, the impact that low-load BFR resistance exercise has on muscle quality and bioenergetics is unknown. The present study will examine the impact of 6 weeks of low-load, single-leg resistance exercise training with or without personalized BFR on measures of muscle mass, strength, quality, and mitochondrial bioenergetics. The investigators will recruit and study up to 30, previously sedentary, healthy, college-aged adults (18-40 years). The investigators will measure muscle mass using Dual Energy X-Ray Absorptiometry and muscle strength and endurance using isokinetic testing. The investigators will normalize knee extensor strength to lower limb lean mass to quantify muscle quality. The investigators will also use near infrared spectroscopy (NIRS) to measure mitochondrial oxidative capacity in the vastus lateralis. Finally, the investigators will measure markers of systemic inflammation and markers of muscle damage using commercially available ELISA assays.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2018-09-21
• Study First Submitted QC: 2018-10-25
• Study First Posted: 2018-10-29
• Study Start: 2019-01-28
• Primary Completion: 2023-07-01
• Study Completion: 2023-07-01
• Status Verified: 2023-08
• Last Update Submitted: 2023-08-14
• Last Update Posted: 2023-08-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Low Load Resistance Exercise + BFR	Low Load Resistance Exercise + BFR	Subjects allocated to Low Load Resistance Exercise + BFR will undergo 6 weeks of single-legged low load (25%) resistance exercise plus blood flow restriction. Their contralateral leg will serve as within subject control.
Low Load Resistance Exercise	Low Load Resistance Exercise	Subjects allocated to Low Load Resistance Exercise will undergo 6 weeks of single-legged low load (25%) resistance exercise. Their contralateral leg will serve as within subject control.

Primary Outcome Measures

Name	Time	Method
Changes in Mitochondrial Oxidative Capacity	Changes from baseline (pre-training) to follow-up (about 48-72 hours post-training).	Changes in mitochondrial oxidative capacity will be measured using near infrared spectroscopy (NIRS).

Secondary Outcome Measures

Name	Time	Method
Changes in Muscle Strength measured using Isokinetic Dynamometry	Changes from baseline (pre-training) to follow-up (about 48-72 hours post-training)	Changes in muscle strength measured using isokinetic dynamometry
Changes in Muscle Endurance measured using Isokinetic Dynamometry	Changes from baseline (pre-training) to follow-up (about 48-72 hours post-training)	Changes in muscle endurance measured using isokinetic dynamometry
Changes in Muscle Mass measured by Dual Energy X-ray Absorptiometry	Changes from baseline (pre-training) to follow-up (about 48-72 hours post-training)	Changes in muscle mass will be measured by Dual Energy X-ray Absorptiometry

Trial Locations

Locations (1)

Lousiana State University; 🇺🇸 Baton Rouge, Louisiana, United States