Effects of Serum From Hepatocellular Carcinoma Surgery Patients Under Different Anesthetic Methods on Hepatic Cancer Cell Malignancy in Vitro
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- cell proliferation
研究概览
简要总结
Numerous studies found that anesthetic methods can influence the recurrence of tumor and the overall survival of patients after primary cancer surgery. Radiofrequency (RF) ablation is now widely used in clinic for treatment of hepatocellular carcinoma (HCC). Currently, diverse anesthetic methods, including general anesthesia (GA), epidural anesthesia and local anesthesia (LA), are used for RF ablation surgery. Using serum from HCC surgery patients randomized to receive either GA or LA during surgery, we investigated the effects of anesthetic methods on proliferation, migration and metastasis in HepG2 hepatic cancer cells in vitro.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •patients with a single primary liver tumor of 3cm or smaller, who are scheduled for RF ablation surgery
排除标准
- •less than 18 or more than 65 years old;
- •ASA Physical Status 4 or greater;
- •previous surgery in liver (including radiofrequency ablation);
- •severe systemic disease (heart, lung, kidney, or immune system);
- •INR>1.5 or platelet count <45,000 cells/mm3;
- •a history of addiction to opioids;
- •with known extension beyond the liver;
- •Child-Pugh Class C.
结局指标
主要结局
cell proliferation
时间窗: 1 hour post-surgery
HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA for 24h and cell proliferation will be measured
次要结局
- cell metastasis(1 hour post-surgery)
- cell migration(1 hour post-surgery)