Effects of Anesthetic Methods on Hepatic Cancer Cell Malignancy

Not Applicable

Completed

• Conditions: Hepatocellular Carcinoma
• Interventions: Procedure: anesthetic methods

• Registration Number: NCT03594188
• Lead Sponsor: RenJi Hospital

Brief Summary

Numerous studies found that anesthetic methods can influence the recurrence of tumor and the overall survival of patients after primary cancer surgery. Radiofrequency (RF) ablation is now widely used in clinic for treatment of hepatocellular carcinoma (HCC). Currently, diverse anesthetic methods, including general anesthesia (GA), epidural anesthesia and local anesthesia (LA), are used for RF ablation surgery. Using serum from HCC surgery patients randomized to receive either GA or LA during surgery, we investigated the effects of anesthetic methods on proliferation, migration and metastasis in HepG2 hepatic cancer cells in vitro.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2018-07-11
• Study First Submitted QC: 2018-07-19
• Study First Posted: 2018-07-20
• Study Start: 2018-07-23
• Primary Completion: 2019-07-31
• Study Completion: 2019-12-31
• Status Verified: 2020-04
• Last Update Submitted: 2020-04-03
• Last Update Posted: 2020-04-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• patients with a single primary liver tumor of 3cm or smaller, who are scheduled for RF ablation surgery

Exclusion Criteria

• less than 18 or more than 65 years old;
• ASA Physical Status 4 or greater;
• previous surgery in liver (including radiofrequency ablation);
• severe systemic disease (heart, lung, kidney, or immune system);
• INR>1.5 or platelet count <45,000 cells/mm3;
• a history of addiction to opioids;
• with known extension beyond the liver;
• Child-Pugh Class C.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
local anesthesia	anesthetic methods	Patients in this group will have RF ablation for treatment of HCC under local anesthesia.
general anesthesia	anesthetic methods	Patients in this group will have RF ablation for treatment of HCC under general anesthesia.

Primary Outcome Measures

Name	Time	Method
cell proliferation	1 hour post-surgery	HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA for 24h and cell proliferation will be measured

Secondary Outcome Measures

Name	Time	Method
cell metastasis	1 hour post-surgery	HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA and cell metastasis will be measured
cell migration	1 hour post-surgery	HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA and cell migration will be measured

Trial Locations

Locations (1)

Renji Hospital affliated to Shanghai Jiaotong University School of Medicine; 🇨🇳 Shanghai, Shanghai, China