The Effect of Probiotic Intake on Intestinal Permeability of Healthy Adults
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Intestinal Barrier Function
- Sponsor
- University of Florida
- Enrollment
- 39
- Locations
- 1
- Primary Endpoint
- Gastroduodenal Permeability
- Status
- Completed
- Last Updated
- 6 years ago
Overview
Brief Summary
In this double blind, crossover study participants will take a placebo for 3 weeks each. Gut permeability will be assessed weekly using food-grade sugar molecules. On the second week, participants will take aspirin, which will make their intestine permeable to the sugars. Participants will be asked to provide urine and stool samples to assess gut permeability and microbial communities. No change in permeability to the small sugar probes is anticipated with the probiotic.
Investigators
Eligibility Criteria
Inclusion Criteria
- Not provided
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Gastroduodenal Permeability
Time Frame: Week 2 of each intervention
The primary outcome is the difference between the changes in gastroduodenal permeability induced by aspirin challenge with the probiotic versus the placebo. Gastroduodenal permeability will be assessed while on the placebo and probiotic interventions by measuring urinary sucrose in the 0 to 5-hour urine collection of the sugar probe permeability test. Change will be calculated as the urinary sucrose concentration after aspirin challenge (week 2 of each intervention) minus the concentration from the prior week (i.e., 1 wk on intervention with no aspirin challenge).
Secondary Outcomes
- Whole Gut Permeability(Weeks 1 and 2 on each intervention)
- Small Intestinal Permeability(Weeks 1 and 2 on intervention)
- Colonic Permeability(Weeks 1 and 2 on intervention)