Evaluate the Influence of Different Levels of Vitamin D on IVF -ICSI Cycles. We Will Evaluate as Well the Influence of Increased Estradiol Level in IVF-ICSI Cycles on Peripheral Vitamin D.

All patients who require IVF treatment will be eligible for our study; even those who are already known for Glucose intolerance. Those patients being treated for this condition will not have their treatment affected in any way by our study, other than having one additional blood test on the day that they present for their down-regulation ultrasound. They will not be required to give a greater amount of blood, since vitamin D measurements can be determined from the same blood drawn routinely for estradiol (E2) levels.

Those patients who consent to participate in our study will have vitamin D levels drawn at each visit, but will not be required to present more often than otherwise required by their treating physician. Given that the only intervention is one additional blood sample, we do not foresee any side-effect or risks. Results of these tests will not have any impact on patients' IVF treatment protocols, as the test results will not be disclosed until after their treatment has ended, whether in failure or in pregnancy.

Patients' medical history, results of blood tests and the details of their IVF treatment protocol will be entered into a database, which will then be analyzed using parametric or non-parametric tests when appropriate, as required using SPSS. All blood tests levels will be drawn and analyzed in the McGill Reproductive Center's laboratory using a single immunoassay run. Measuring all samples at the same time reduces the possibility of inter-assay variability, reducing the sensitivity of the study. The sample size of experimental patients (with 10% margins of error and 95% confidence limits) is 66 women for a control group of 200. These numbers are easily achievable with the current activity of the McGill Reproductive Centre.