COVID-ASTER Obesity and COVID-19: AdipoSe TissuE Responses to SARS-CoV-2 infectio

Recruiting

Conditions: COVID-19
SARS-CoV-2
10047438

Registration Number: NL-OMON51186

Lead Sponsor: niversitair Medisch Centrum Groningen

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: Not specified

Target Recruitment: 36

Inclusion Criteria

Intervention group (Obese)
Healthy participants who give informed consent to participate in this study
that are male and female, between aged between 18 and 65 years old with a BMI
above 40.

Control group (Lean)
The control group will consist of participants that give informed consent to
participate in this study, male and female aged between 18 and 65 years old,
without comorbidities, no cancer and a BMI of 25 or less.

Exclusion Criteria

For both groups: Diabetes mellitus, hypertension, metabolic syndrome, asthma,
immune disease such as Crohn colitis, rheumatic disease, cancer, use of
soft/hard drugs or smoking. Previous COVID-19 infection.

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Main study parameters/endpoints :<br /><br>1. To determine if adipose tissue is permissive to infection by plaque assay<br /><br>and qPCR of viral RNA.<br /><br>2. To determine the mRNA and protein expression of SARS-CoV-2 receptors such as<br /><br>ACE2 and to perform blocking experiments to elucidate the entry receptor.<br /><br>3. To determine if there is an exaggerated inflammatory response upon<br /><br>SARS-CoV-2 infection by examining the mRNA levels of inflammatory mediators and<br /><br>adipokines. As well as the protein levels of adipose-tissue derived factors and<br /><br>inflammatory mediators released upon SARS-CoV-2 infection in culture medium by<br /><br>Luminex analysis. </p><br>

Secondary Outcome Measures