ACTH Stability on Whole Blood

Not Applicable

• Conditions: ACTH
• Interventions: Biological: Blood sampling

• Registration Number: NCT04266587
• Lead Sponsor: University Hospital, Rouen

Brief Summary

ACTH is a peptide secreted by pituitary gland and plays an important role in regulating cortisol secretion. ACTH is determined in plasma by immunoassays using specific antibodies. Its determination is difficult because of instability in whole blood. Several factors which influence ACTH stability in blood before analysis have been identified: temperature, hemolysis, time to centrifugation and presence of protease inhibitors. Published results on ACTH whole blood stability seem contradictory.

The objective of this study is to evaluate the effect of aprotinin in 10 healthy volunteers. ACTH measurements will be performed on cobas e602 (Roche Diagnostics, Mannheim, Germany).

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2020-02-10
• Study First Submitted QC: 2020-02-10
• Study First Posted: 2020-02-12
• Status Verified: 2020-06
• Last Update Submitted: 2020-06-26
• Last Update Posted: 2020-06-29
• Study Start: 2020-07
• Primary Completion: 2020-11
• Study Completion: 2020-11

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

• Adult patient aged 18 to 60,

Exclusion Criteria

• Adult with insufficient venous capital for blood collection

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Healthy volunteers	Blood sampling	blood sampling is done on healthy volunteers

Primary Outcome Measures

Name	Time	Method
ACTH concentration in plasma	8 hours

Trial Locations

Locations (1)

Rouen University Hospital; 🇫🇷 Rouen, France