Comparison of Mouse Gingival Fibroblast Attachment on Periodontally involved Human Root Surfaces after Mechanical Debridement Alone or Followed by Treatment with either Citric acid, Tetracycline hydrochloride or Nd: YAG irradiation through SEM analysis and MTT Assay .
Not Applicable
Completed
- Registration Number
- CTRI/2018/12/016545
- Lead Sponsor
- Dr Ashwini Samaleti
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 10
Inclusion Criteria
1.Periodontally involved single rooted teeth with loss of attachment more than 5mm with hopeless prognosis indicated for extraction
2.Subjects who has not undergone any non-surgical / surgical periodontal therapy
3.Systemically healthy subjects aged above 18 years
Exclusion Criteria
1.Teeth with root caries.
2.Teeth with prosthetic restoration.
3.Teeth with restoration on root surface.
4.Teeth with endodontic treatment.
5.Teeth with fracture.
6.Teeth with attrition.
7.Teeth with abrasion.
8.Teeth with erosion.
9.Teeth with abfraction.
10.Teeth with internal or external resorption.
11.Teeth with developmental anomalies.
12.Teeth having received vital / non-vital bleaching
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Scanning Electron Microscopic analysis at 1500x magnification for murine gingival fibroblast <br/ ><br>adhesion on treated root surfacesTimepoint: Assessment post culture at 96 hrs.
- Secondary Outcome Measures
Name Time Method MTT assay for murine gingival fibroblast viability on treated root surfacesTimepoint: Assessment post culture at 24 hrs, 42 hrs, 72 hrs,96 hrs.