Regeneration of Bronchial Epithelium During Chronic Obstructive Pulmonary Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- CHU de Reims
- 入组人数
- 25
- 试验地点
- 1
- 主要终点
- Mean speed of wound closure (MSWC) of wounded bronchial epithelial cell culture obtained from COPD and control patients
研究概览
简要总结
Airway epithelium integrity is essential to maintain its role of mechanical and functional barrier. Recurrent epithelial injuries require a complex mechanism of repair to restore its integrity. In chronic obstructive pulmonary disease (COPD), an abnormal airway epithelial repair may participate in airway remodelling. The objective was to determine if airway epithelial wound repair of airway epithelium is abnormal in COPD.
详细描述
Patients scheduled for lung resection were prospectively recruited. Demographic, clinical data and pulmonary function tests results were recorded. Emphysema was visually scored and histological remodelling features were noted. Primary bronchial epithelial cells (BEC) were extracted and cultured for wound closure assay. The investigators determined the mean speed of wound closure (MSWC) and cell proliferation index, matrix metalloprotease (MMP)-2, MMP-9 and cytokines levels in supernatants of BEC 18 hours after cell wounding. In a subset of patients, bronchiolar epithelial cells were also cultured for wound closure assay for MSWC analyze.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 40 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •6 Patients scheduled for lung resection for cancer
排除标准
- •Patients with asthma, cystic fibrosis, bronchiectasis or pulmonary fibrosis were excluded
结局指标
主要结局
Mean speed of wound closure (MSWC) of wounded bronchial epithelial cell culture obtained from COPD and control patients
时间窗: hour 18
次要结局
- Matrix metalloprotease (MMP)-9 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.(hour 18)
- Cytokines levels in supernatants of bronchial epithelial cells 18 hours after cell wounding.(hour 18)
- Cell proliferation index in supernatants of bronchial epithelial cells 18 hours after cell wounding.(hour 18)
- Matrix metalloprotease (MMP)-2 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.(hour 18)