Regeneration of Bronchial Epithelium During Chronic Obstructive Pulmonary Disease

Completed

• Conditions: COPD
• Interventions: Other: bronchial epithelial cell culture

• Registration Number: NCT02873988
• Lead Sponsor: CHU de Reims

Brief Summary

Airway epithelium integrity is essential to maintain its role of mechanical and functional barrier. Recurrent epithelial injuries require a complex mechanism of repair to restore its integrity. In chronic obstructive pulmonary disease (COPD), an abnormal airway epithelial repair may participate in airway remodelling. The objective was to determine if airway epithelial wound repair of airway epithelium is abnormal in COPD.

Detailed Description

Patients scheduled for lung resection were prospectively recruited. Demographic, clinical data and pulmonary function tests results were recorded. Emphysema was visually scored and histological remodelling features were noted. Primary bronchial epithelial cells (BEC) were extracted and cultured for wound closure assay. The investigators determined the mean speed of wound closure (MSWC) and cell proliferation index, matrix metalloprotease (MMP)-2, MMP-9 and cytokines levels in supernatants of BEC 18 hours after cell wounding. In a subset of patients, bronchiolar epithelial cells were also cultured for wound closure assay for MSWC analyze.

Study Timeline

• Study Start: 2012-03
• Primary Completion: 2013-12
• Study Completion: 2014-05
• Status Verified: 2016-08
• Study First Submitted: 2016-08-10
• Study First Submitted QC: 2016-08-16
• Last Update Submitted: 2016-08-16
• Study First Posted: 2016-08-22
• Last Update Posted: 2016-08-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 25

Inclusion Criteria

6 Patients scheduled for lung resection for cancer

Exclusion Criteria

• Patients with asthma, cystic fibrosis, bronchiectasis or pulmonary fibrosis were excluded

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
patients without COPD	bronchial epithelial cell culture	patients without COPD
patients with COPD	bronchial epithelial cell culture	patients with COPD

Primary Outcome Measures

Name	Time	Method
Mean speed of wound closure (MSWC) of wounded bronchial epithelial cell culture obtained from COPD and control patients	hour 18

Secondary Outcome Measures

Name	Time	Method
Matrix metalloprotease (MMP)-9 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.	hour 18
Cytokines levels in supernatants of bronchial epithelial cells 18 hours after cell wounding.	hour 18
Cell proliferation index in supernatants of bronchial epithelial cells 18 hours after cell wounding.	hour 18
Matrix metalloprotease (MMP)-2 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.	hour 18

Trial Locations

Locations (1)

Chu de Reims; 🇫🇷 Reims, France