MICrobiota, KEY between Mother and Offspring USsing SEquencing techniques

Completed

• Conditions: NEC; necrotising enterocolitis; 10017969

• Registration Number: NL-OMON49693
• Lead Sponsor: niversitair Medisch Centrum Groningen

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 19 August 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 150

Inclusion Criteria

preterm children born before 30 weeks' gestational age, and/or birth weights
below 1000 grams.

Exclusion Criteria

Other abdominal diseases, e.g. abdominal wall defects or congenital intestinal
atresias.

Study & Design

• Study Type: Observational non invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Development of necrotising enterocolitis</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Neonatal: fecal microbiota, fecal human DNA methylation pattern (shedded<br /><br>enterocytes and lymphocytes), fecal bile salts acids, fecal SCFA and iAF, iAF<br /><br>expression in the margins of the resection specimens, urine metabolome,<br /><br>cerebral and intestinal tissue oxygen saturation (as measured with NIRS), peak<br /><br>systolic flow velocity and the mean end-diastolic flow velocity of the superior<br /><br>mesenteric artery and a branch of the medial cerebral artery assessment using<br /><br>Echo Doppler, serum IMA, serum (i)AF, T1/T2 cytokine ratio in umbilical cord<br /><br>blood, iAF in resection specimens<br /><br><br /><br>Mother: microbiota in mother*s milk, vaginal, perineal and rectal microbiota;<br /><br>amnionic fluid in case of caesarian,validated diet questionnaire ('Wat een<br /><br>Nederland' questionnaire as used by RIVM)</p><br>