Impact of a High Saturated Fat Diet on Fasted Systemic and White Adipose Tissue Inflammatory Responses
- Conditions
- ObesityCardiovascular DiseasesInflammationInflammatory Response
- Interventions
- Other: High Fat Diet
- Registration Number
- NCT03569189
- Lead Sponsor
- Loughborough University
- Brief Summary
This study will investigate the effect of a 7-day westernised high-fat (65% of kilocalories), high-calorie (150% of requirements) diet on markers of inflammation in the blood and white adipose tissue. Participants will firstly complete a 3-day weight maintenance phase (Days 1-3) before completing a 7-day high fat diet intervention (Days 4-10). On days 4 and 11 participants will complete a laboratory visit where anthropometric measurements, blood and adipose samples will be collected.
The investigators hypothesise that consuming a high-fat, high-calorie diet for 7 days will alter the inflammatory responses in white adipose tissue and will induce metabolic endotoxaemia / systemic inflammation.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 15
Not provided
Not provided
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description High Fat Diet High Fat Diet Participants will consume a high-fat, high-calorie diet for 7 days (i.e. westernised diet) following a 3-day weight maintenance diet. Measurements will be made pre- and post-high fat diet intervention.
- Primary Outcome Measures
Name Time Method Protein expression (content and phosphorylation) of key markers of metabolic inflammation in white adipose tissue (for example assessment of NFKB/IKBa total protein and phosphorylation by western blot analysis) Change between pre- and post-high fat diet (assessed on days 4 and 11) This will be assessed following the collection of fasted white adipose tissue samples
- Secondary Outcome Measures
Name Time Method Systemic Markers of Inflammation (for example CRP, TNFa and IL-6 concentrations, determined by spectrophotometric assay/ ELISA) Change between pre- and post-high fat diet (assessed on days 4 and 11) Assessed following the collection of fasted blood samples
Fasting Serum Lipid Profile (for example total, HDL and LDL cholesterol, TAG and NEFA concentrations, measured using a spectrophotometric assay) Change between pre- and post-high fat diet (assessed on days 4 and 11) Assessed following the collection of fasted blood samples
Immune Cell Populations in the Blood Change between pre- and post-high fat diet (assessed on days 4 and 11) Assessed following the collection of fasted blood samples
Gene expression of key markers of metabolic inflammation in white adipose tissue Change between pre- and post-high fat diet (assessed on days 4 and 11) This will be assessed following the collection of fasted white adipose tissue samples
Anthropometric Measurements (for example height and weight that will be aggregated to report BMI in kg/m^2) Change between pre- and post-high fat diet (assessed on days 4 and 11) Measured using standard equipment
Physical Activity Habitual physical activity will be assessed for 3 days prior to commencing the high-fat diet intervention (days 1-3) and during the final 3 days of the intervention (days 8-10) Assessed using Actigraph accelerometers
Fasting Serum Markers of Insulin Resistance (for example insulin and glucose concentrations, determined using an ELISA/ spectrophotometric assay) Change between pre- and post-high fat diet (assessed on days 4 and 11) Assessed following the collection of fasted blood samples
Systemic Markers of Metabolic Endotoxemia (for example LBP and sCD14 concentrations, determined using an ELISA) Change between pre- and post-high fat diet (assessed on days 4 and 11) Assessed following the collection of fasted blood samples
Trial Locations
- Locations (1)
Loughborough University
🇬🇧Loughborough, Leicestershire, United Kingdom